In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines
- 823 Downloads
In this study, we present in vitro cytotoxicity of iron oxide (Fe3O4) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5–500 μg/ml), incubation time (18–96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 μg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe3O4 and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe3O4 is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe3O4 exhibited excellent stability compared with Feridex for a prolonged incubation time.
KeywordsWater-dispersible Fe3O4 and MnO nanoparticle Magnetic resonance imaging contrast agents Cytotoxicity Nanotoxicology
- 5.Kagan VE, Bayir H, Shvedova AA (2005) Nanomedicine and nanotoxicology: two sides of the same coin. Nanomedicine 1:313–316Google Scholar
- 7.Linkov I, Satterstrom FK, Corey LM (2008) Nanotoxicology and nanomedicine: making hard decisions. Nanomedicine 4:167–171Google Scholar
- 20.Hayes AW (1994) Principles and methods of toxicology, 3rd edn. Raven Press, New York, pp 1231–1258Google Scholar
- 39.Konorev EA, Zhang H, Joseph J, Kennedy MC, Kalyanaraman B (2000) Bicarbonate exacerbates oxidative injury induced by antitumor antibiotic doxorubicin in cardiomyocytes. Am J Physiol 279:H2424–H2430Google Scholar