In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines

  • Jong Young Choi
  • Su Hee Lee
  • Hyon Bin Na
  • Kwangjin An
  • Taeghwan Hyeon
  • Tae Seok Seo
Original Paper


In this study, we present in vitro cytotoxicity of iron oxide (Fe3O4) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5–500 μg/ml), incubation time (18–96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 μg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe3O4 and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe3O4 is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe3O4 exhibited excellent stability compared with Feridex for a prolonged incubation time.


Water-dispersible Fe3O4 and MnO nanoparticle Magnetic resonance imaging contrast agents Cytotoxicity Nanotoxicology 


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jong Young Choi
    • 1
  • Su Hee Lee
    • 1
  • Hyon Bin Na
    • 2
  • Kwangjin An
    • 2
  • Taeghwan Hyeon
    • 2
  • Tae Seok Seo
    • 1
  1. 1.Department of Chemical and Biomolecular Engineering (BK21 Program)Institute for the BioCentury, KAISTDaejeonKorea
  2. 2.National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological EngineeringSeoul National UniversitySeoulKorea

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