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Enhanced immobilization of hexa-arginine-tagged esterase on gold nanoparticles using mixed self-assembled monolayers

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Abstract

Mixed self-assembled monolayers (MSAMs) composed of diverse ligands offer a mechanism for the specific binding of biomolecules onto solid surfaces. In this study, we examined the formation of MSAMs on gold nanoparticles (AuNPs) and the immobilization of hexa-arginine-tagged esterase (Arg6-esterase) on the surfaces of the resulting particles. The functionalization of AuNPs with MSAMs was achieved by introducing a mixture of tethering and shielding ligands into an AuNP solution. The formation of self-assembled monolayers (SAMs) on the AuNP surface was characterized by UV/visible spectroscopy, transmission electron microscopy, and Fourier-transform infrared spectroscopy. Arg6-esterase was immobilized in a highly specific manner onto AuNPs treated with mixed SAMs (MSAM–AuNPs) by providing a shielding ligand which reduce the non-specific adsorption of enzymes caused by hydrophobic interaction compared to AuNPs treated with single-component SAMs (SSAM–AuNPs). Moreover, Arg6-esterase immobilized on MSAM–AuNPs showed substantially enhanced catalytic activity up to an original activity compared to that on SSAM–AuNPs (58%).

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Acknowledgments

This work was supported by grant no. RTI04-03-06 from the Regional Technology Innovation Program of the Ministry of Knowledge and Economy (MKE), and the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, 111 Gwahangno, Yuseong-gu, Daejeon, 305-600, South Korea

    Jinyoung Jeong, Chang Soo Lee, Sang Jeon Chung & Bong Hyun Chung

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  1. Jinyoung Jeong
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  2. Chang Soo Lee
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  3. Sang Jeon Chung
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  4. Bong Hyun Chung
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Correspondence to Bong Hyun Chung.

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Jeong, J., Lee, C.S., Chung, S.J. et al. Enhanced immobilization of hexa-arginine-tagged esterase on gold nanoparticles using mixed self-assembled monolayers. Bioprocess Biosyst Eng 33, 165–169 (2010). https://doi.org/10.1007/s00449-009-0353-6

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  • DOI: https://doi.org/10.1007/s00449-009-0353-6

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