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Chiral resolution of phenylalanine by d-Phe imprinted membrane considering rejection property

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Abstract

An enantio-selective d-Phe imprinted P(AA-co-AN) membrane was prepared using the wet-phase inversion method. The membrane not only selectively adsorbed phenylalanine but also rejected it with a rejection selectivity of 0.82–0.64 and 0.91–0.63 during the filtration of 100 and 10 ppm (g m−3) racemate solutions, respectively. The fluxes of d-Phe and l-Phe during filtration of 10 ppm racemate solution were 0.0077–0.0229 and 0.0064–0.0208 mg m−2 s−1, respectively, and the fluxes of d-Phe and l-Phe during filtration of 100 ppm racemate solution were 0.1287–0.2522 and 0.1174–0.2458 mg m−2 s−1, respectively. The adsorption selectivity was higher at low concentration. The adsorption selectivities varied from 1.11 to 1.65 and from 1.64 to 2.78 during filtration of 100 and 10 ppm racemate solutions, respectively. In respect to desorption, the fractional difference between d-Phe and l-Phe in the recovered solution from 10 ppm was higher than that from 100 ppm.

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Abbreviations

A :

The surface area of membrane (m2)

C o :

Concentration of d- or l-Phe in the feed solution (mg m−3)

C p :

Concentration of d- or l-Phe in permeate (mg m−3)

C PD :

Concentration of d-Phe in the permeate (mg m−3)

C PL :

Concentration l-Phe in the permeate (mg m−3)

C r :

Concentrations of d- or l-Phe in the retentate (mg m−3)

J Phe :

Flux of d- or l-Phe (mg m−2 s−1)

J W :

Flux of solution (m s−1)

P :

Permeability coefficient (m2 s−1)

P D :

Permeability coefficient of the d-Phe (m2 s−1)

P L :

Permeability coefficient of the l-Phe (m2 s−1)

Q p :

Amount of the template adsorbed on the membrane (mg g−1)

Q D :

Amount of the counter enantiomer adsorbed on the membrane (mg g−1)

R :

Rejection of the d- or l-Phe (%)

R RD :

Rejection of d-Phe (%)

R RL :

Rejection of l-Phe (%)

S DP :

Uptake value of the d-Phe (mg)

S LP :

Uptake value of l-Phe (mg)

T :

The required time for a solution to pass through the membrane (s)

T :

Time (in seconds) required water to pass through the membrane (s)

V O :

Volumes of feed solution (m−3)

V R :

Volumes of retentate (m−3)

V W :

Volume of the permeate in (m3)

α:

Adsorption selectivity of the membrane

α d :

Adsorption selectivity of the d-Phe imprinted membrane

αR :

Rejection selectivity of the membrane

α PS :

Permselectivity of the d-Phe imprinted membrane

∂:

Thickness of membrane (m)

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Acknowledgment

The authors are indebted to the Center for Advanced Bioseparation Technology for supporting this research.

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Authors and Affiliations

  1. School of Chemical and Materials Engineering, National University of Science and Technology, H-12 Campus, Islamabad, Pakistan

    Noaman Ul-Haq

  2. Department of Chemical Engineering, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, Korea

    Joong Kon Park

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  1. Noaman Ul-Haq
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  2. Joong Kon Park
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Correspondence to Joong Kon Park.

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Ul-Haq, N., Park, J.K. Chiral resolution of phenylalanine by d-Phe imprinted membrane considering rejection property. Bioprocess Biosyst Eng 33, 79–86 (2010). https://doi.org/10.1007/s00449-009-0352-7

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  • DOI: https://doi.org/10.1007/s00449-009-0352-7

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