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Cocultivation of Lactococcus lactis and Teredinobacter turnirae for biological chitin extraction from prawn waste

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Abstract

In this study, mainly biological treatment of prawn waste for chitin production was investigated. Lactic acid and protease fermentations were applied to extract chitin from prawn waste in the presence of various glucose concentrations. The results obtained were also compared with those of chemical method which was consisted of first mineral removal and then protein removal sequence. Different strategies were applied using lactic acid producing bacterium, Lactococcus lactis, and a protease producer, marine bacterium Teredinobacter turnirae. Both bacteria were first cultivated individually and then cofermented. In their individual cultivation, L. lactis removed the inorganic materials efficiently, while T. turnirae performed better in deproteinization process. Cofermentation of both bacteria was also conducted using three different protocols. The highest process yield (95.5%) was obtained when T. turnirae was first inoculated. Although the extraction of chitin by biological treatment was incomplete compared to the chemical method, the biological treatment employed here could still be considered as an alternative method in a more environmentally benign approach.

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References

  1. Simpson BK, Gagne N, Simpson MV (1994) Bioprocessing of chitin and chitosan. In: Martin AM (ed) Fisheries processing: biotechnological applications. Chapman and Hall, London

    Google Scholar 

  2. Kumar R (2000) A review of chitin and chitosan applications. React Funct Polym 46:1–27

    Article  CAS  Google Scholar 

  3. Qunvu L, Embree H, Smith P, Payne GF (2002) Utilizing renewable resources to create functional polymers: chitosan-based associative thickener. Environ Sci Technol 36:3446–3454

    Article  Google Scholar 

  4. Khor E, Lim LY (2003) Implantable applications of chitin and chitosan. Biomaterials 24:2339–2349

    Article  CAS  Google Scholar 

  5. Rinaudo M (2006) Chitin and chitosan: properties and applications. Progr Polym Sci 31:603–632

    Article  CAS  Google Scholar 

  6. Beaney P, Lizardi-Mendoza J, Healy M (2005) Comparison of chitins produced by chemical and bioprocessing methods. J Chem Technol Biotechnol 80:145–150

    Article  CAS  Google Scholar 

  7. Muzarelli RAA, Peter MG (1997) Chitin handbook. European Chitin Society, Italy

    Google Scholar 

  8. Abdou ES, Nagy KSA, Elsabee MZ (2007) Extraction and characterization of chitin and chitosan from local sources. Biores Technol 99:1359–1367

    Article  Google Scholar 

  9. No HK, Meyers SP, Lee KS (1989) Isolation and characterization of chitin from crawfish shell waste. J Agric Food Chem 37:575–579

    Article  CAS  Google Scholar 

  10. Healy M, Green A, Healy A (2003) Bioprocessing of marine crustacean shell waste. Acta Biotechnol 23:151–160

    Article  CAS  Google Scholar 

  11. Teng WL, Khor E (2001) Concurrent production of chitin from shrimp shells and fungi. Carbohydr Res 332:305–316

    Article  CAS  Google Scholar 

  12. Rao MS, Stevens WF (2005) Chitin production by Lactobacillus fermentation of shrimp biowaste in a drum reactor and its chemical conversion to chitosan. J Chem Technol Biotechnol 80:1080–1087

    Article  CAS  Google Scholar 

  13. Greene RV (1994) Challenges from the sea: marine shipworms and their symbiotic bacterium. Soc Ind Microbiol News 44:51–59

    Google Scholar 

  14. Ahuja SK, Ferreira GM, Moreira AR (2004) Application of Plackett-Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium. Biotechnol Bioeng 85:666–675

    Article  CAS  Google Scholar 

  15. Rao MS, Stevens WF (1997) Processing parameters in scale-up of Lactobacillus fermentation of shrimp biowaste. In: Domard A, Roberts GAF, Varum KM (eds) Jacques Andre advances in chitin science. Lyon, France

    Google Scholar 

  16. Greene RV, Cotta MA, Griffin HL (1989) A novel, symbiotic bacterium isolated from marine shipworm secretes proteolytic activity. Curr Microbiol 19:353–356

    Article  CAS  Google Scholar 

  17. Jung WJ, Jo GH, Kuk JH, Kim KY, Park RD (2006) Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serretia marcescens FS-3. Appl Microbiol Biotechnol 71:234–237

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported through a research grant from The Scientific and Technological Research Council of Turkey (TUBITAK) with a project number of 106M241.

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Authors and Affiliations

  1. Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, Kumamoto, Japan

    Ozhan Aytekin

  2. Bioengineering Department, Faculty of Engineering, Ege University, EBILTEM Hall, Bornova, 35100, Izmir, Turkey

    Murat Elibol

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  1. Ozhan Aytekin
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  2. Murat Elibol
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Correspondence to Murat Elibol.

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Aytekin, O., Elibol, M. Cocultivation of Lactococcus lactis and Teredinobacter turnirae for biological chitin extraction from prawn waste. Bioprocess Biosyst Eng 33, 393–399 (2010). https://doi.org/10.1007/s00449-009-0337-6

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  • DOI: https://doi.org/10.1007/s00449-009-0337-6

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