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Enhanced fructooligosaccharides and inulinase production by a Xanthomonas campestris pv. phaseoli KM 24 mutant

Abstract

Xanthomonas campestris pv phaseoli produced an extracellular endoinulinase (9.24 ± 0.03 U mL−1) in an optimized medium comprising of 3% sucrose and 2.5% tryptone. X. campestris pv. phaseoli was further subjected to ethylmethanesulfonate mutagenesis and the resulting mutant, X. campestris pv. phaseoli KM 24 demonstrated inulinase production of 22.09 ± 0.03 U mL−1 after 18 h, which was 2.4-fold higher than that of the wild type. Inulinase production by this mutant was scaled up using sucrose as a carbon source in a 5-L fermenter yielding maximum volumetric (21,865 U L−1 h−1) and specific (119,025 U g−1 h−1) productivities of inulinase after 18 h with an inulinase/invertase ratio of 2.6. A maximum FOS production of 11.9 g L−1 h−1 and specific productivity of 72 g g−1 h−1 FOS from inulin were observed in a fermenter, when the mutant was grown on medium containing 3% inulin and 2.5% tryptone. The detection of mono- and oligosaccharides in inulin hydrolysates by TLC analysis indicated the presence of an endoinulinase. This mutant has potential for large-scale production of inulinase and fructooligosaccharides.

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Acknowledgments

This study was supported by grants from the National Research Foundation, Republic of South Africa. The authors are thankful to Prof. Bernard Prior, University of Stellenbosch, South Africa, for his valuable suggestions and critical evaluation of the manuscript.

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Correspondence to Manimaran Ayyachamy.

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Naidoo, K., Ayyachamy, M., Permaul, K. et al. Enhanced fructooligosaccharides and inulinase production by a Xanthomonas campestris pv. phaseoli KM 24 mutant. Bioprocess Biosyst Eng 32, 689–695 (2009). https://doi.org/10.1007/s00449-008-0293-6

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  • DOI: https://doi.org/10.1007/s00449-008-0293-6

Keywords

  • EMS
  • Fructooligosaccharides
  • Inulinase
  • Invertase
  • Mutagenesis
  • Xanthomonas campestris pv. phaseoli