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Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

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Abstract

The purification of clavulanic acid (CA), which is an important β-lactam antibiotic produced by submerged cultivation of Streptomyces clavuligerus, was studied through the use of phosphate and polyethylene glycol-based aqueous two-phase systems. The parameters’ effect on the yield and purification was evaluated through an experimental design and the preliminary results showed that the polyethylene molecular mass and tie-line length and phase volume ratio exerted the strongest effect on the yield and distribution coefficient in the range tested. In addition, the response surface methodology was used to optimize the distribution coefficient, yield, and purification factor. The optimal conditions of yield and purification factor are in the regions where polyethylene has a low molecular mass, pH close to the isoelectric point, and lower top phase volume. A 100% yield and a 1.5-fold purification factor are obtained when extracting CA by maximizing the conditions of an aqueous two-phase system.

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Acknowledgments

The authors gratefully acknowledge the financial support of FAPESP (Grants 04/16056-6, and 05/55079-4) and the CNPq scholarship granted to C.S. da Silva (Doctorate)

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal de São Carlos, Bairro Monjolinho, São Carlos, SP, CEP 13565-905, Brazil

    C. S. Silva, E. Bovarotti, C. O. Hokka & Marlei Barboza

  2. Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Rua Monteiro Lobato, Campinas, SP, CEP 13083-862, Brazil

    M. I. Rodrigues

Authors
  1. C. S. Silva
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  2. E. Bovarotti
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  3. M. I. Rodrigues
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  4. C. O. Hokka
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  5. Marlei Barboza
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Corresponding author

Correspondence to Marlei Barboza.

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Silva, C.S., Bovarotti, E., Rodrigues, M.I. et al. Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems. Bioprocess Biosyst Eng 32, 625–632 (2009). https://doi.org/10.1007/s00449-008-0285-6

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  • DOI: https://doi.org/10.1007/s00449-008-0285-6

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