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Kinetic study of mannitol production using cashew apple juice as substrate

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The use of agriculture excess as substrate in industrial fermentations became an interesting alternative to reduce production costs and to reduce negative environmental impact caused by the disposal of these products. In this work, a kinetic study of mannitol production using cashew apple juice as substrate was studied. The carbohydrates of cashew apple juice are glucose and fructose. Sucrose addition favored the yield of mannitol (85%) at the expense of lower productivity. The best results were obtained applying only cashew apple juice as substrate, containing 50 g L−1 of total reducing sugar (28 g L−1 of fructose), yielding 18 g L−1 of mannitol with 67% of fructose conversion into mannitol and productivity of 1.8 g L−1 h−1.

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  1. Debord B, Lefebvere C, Guyot-Hermman AM, Hubert J, Bouche R, Guyot JC (1987) Study of different forms of mannitol: comparative behavior under compression. Drug Dev Ind Pharm 13:1533–1546

    Article  CAS  Google Scholar 

  2. Dwivedi BK (1978) Low caloric and special dietary foods. CRC Press, West Palm Beach

  3. Griffing WC, Lynch MJ (1992) Polyhydric alcohols. In: Furia TE (ed) CRC Handbook of food additives, vol 1, 2nd edn. CRC Press, Cleveland

  4. Wisselink HW, Weusthuis RA, Eggink G, Hugenholtz J, Gorbben JH (2002) Mannitol production by lactic acid bacteria: a review. Int Dairy J 12:151–161

    Article  CAS  Google Scholar 

  5. Mackee M, Kieboom APG, Van Bekkum H (1985) Production methods of d-mannitol. Starch 37:136–141

    Article  Google Scholar 

  6. Vandamme EJ, Van Loo J, De Laprorte A (1987) Dynamics and regulation of sucrose phosphorylase formation in Leuconostoc mesenteroides fermentation. Biotechnol Bioeng 29:8–15

    Article  CAS  Google Scholar 

  7. Grobben GJ, Peters SWPG, Wisselink HW, Weusthuis RA, Hoefnagel MHN, Hugenholtz J, Eggink G (2001) Spontaneous formation of mannitol-producing variant of Leuconostoc peseudomesenteroides grown in the presence of fructose. Ann N Y Acad Sci 67:2867–2870

    CAS  Google Scholar 

  8. Erten H (1998) Metabolism of fructose as an electron acceptor by Leuconostoc mesenteroides. Process Biochem 33:735–739

    Article  CAS  Google Scholar 

  9. Angumeenal AR, Venkappayya D (2005) Artrocarpus heterophyllus: a potential substrate for citric acid biosyntehsis using Aspergillus niger. Lebensm-Wiss u-Technol 38:89–93

    Article  CAS  Google Scholar 

  10. Kuerbanoglu EB (2004) Enhancement of citric acid production with ram horn hydrolysate by Aspergillus niger. Biores Technol 92:97–101

    Article  Google Scholar 

  11. El-Samragy YA, Khorshid MA, Foda MI, Shehata AE (1996) Effect of fermentation conditions on the production of citric acid from cheese whey by Aspergillus niger. Int J Food Microbiol 29:411–416

    Article  CAS  Google Scholar 

  12. Tony WY, Fu XY, Lee SM, Yu J, Wei DZ (2004) Purification of l (+) lactic acid from fermentation broth with paper sludge as cellulosic feedstock using weak anion exchange Amberlite IRA-92. Biochem Eng J 18:89–96

    Article  Google Scholar 

  13. Campos DCP, Santos AS, Wolkoff DB, Matta VM, Cabral LMC, Couri S (2004) Cashew apple juice stabilization by microfiltration. Desalination 148:61–65

    Article  Google Scholar 

  14. Oliveira MEB, Oliveira GSF, Maia GA, Moreira RA (2002) Aminoácidos majoritários no suco de caju: variação ao longo da safra. Rev Brás Fruct 24:133–137

    Google Scholar 

  15. AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Arlington, VA

  16. Honorato TL, Rabelo MC, Gonçalves LRB, Pinto GAS, Rodrigues S (2007) Fermentation of cashew apple juice to produce high added value products. World J Microbiol Biotechnol 23:1409–1415

    Article  CAS  Google Scholar 

  17. Chagas CMA, Honorato TL, Pinto GAS, Maia GA, Rodrigues S (2007) Dextransucrase production using cashew apple juice as substrate:effect of phosphate and yeast extract addition. Bioprocess Biosyst Eng 30:207–215

    Article  CAS  Google Scholar 

  18. Rodrigues S, Lona LMF, Franco TT (2003) Effect of phosphate concentration on production of dextransucrase by Leuconostoc mesenteroides NRRL B512F. Bioprocess Biosyst Eng 23:57–62

    Article  Google Scholar 

  19. Rodrigues S, Lona LMF, Franco TT (2005) The effect of maltose on dextran yield and molecular weight distribution. Bioprocess Biosyst Eng 28:9–14

    Article  CAS  Google Scholar 

  20. Dubois M, Pilles KA, Hamilton PA, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related subs trances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  21. Alsop L (1993) Industrial production of dextran. Prog Ind Microbiol 18:1–44

    Google Scholar 

  22. Dols M, Chrabi W, Remaund-Simeon M, Lindley ND, Monsan PF (1998) Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences on dextransucrase production. Appl Environ Microbiol 63:2159–2165

    Google Scholar 

  23. Salou P, Loubiere P, Pareilleux A (1994) Growth and energetic of Leuconostoc oenos during cometabolism of glucose and fructose. Appl Environ Microbiol 60:1459–1466

    CAS  Google Scholar 

  24. Yun JW, Kim DH (1998) A comparative study of mannitol production by two lactic acid bacteria. J Ferment Bioeng 85:203–208

    Article  CAS  Google Scholar 

  25. Soetaert W, Buchholtz K, Vandamme EJ (1990) Production of d-mannitol and d-lactic acid from starch hydroalysates by fermentation with Leuconostoc mesenteroides. CR Acad Agric Fr 80:119–126

    Google Scholar 

  26. Weyman N, Kiviharju K, Leisola M (2002) High level production of d-mannitol with membrane cell-recycle bioreactor. J Ind Microbiol Biotechnol 29:44–49

    Article  Google Scholar 

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Correspondence to Sueli Rodrigues.

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Fontes, C.P.M.L., Honorato, T.L., Rabelo, M.C. et al. Kinetic study of mannitol production using cashew apple juice as substrate. Bioprocess Biosyst Eng 32, 493–499 (2009).

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