Skip to main content
SpringerLink
Log in

Studies on the activity and stability of immobilized horseradish peroxidase on poly(ethylene terephthalate) grafted acrylamide fiber

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Having been activated with glutaraldehyde, modified poly(ethylene terephthalate) grafted acrylamide fiber was used for the immobilization of horseradish peroxidase (HRP). Both the free HRP and the immobilized HRP were characterized by determining the activity profile as a function of pH, temperature, thermal stability, effect of organic solvent and storage stability. The optimum pH values of the enzyme activity were found as 8 and 7 for the free HRP and the immobilized HRP respectively. The temperature profile of the free HRP and the immobilized HRP revealed a similar behaviour, although the immobilized HRP exhibited higher relative activity in the range from 50 to 60 °C. The immobilized HRP showed higher storage stability than the free HRP.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Similar content being viewed by others

References

  1. Mateo C, Palomo JM, Lorente GF, Guisan JM, Lafuente RF (2007) Enzyme Microb Technol 40:1451

    Article  CAS  Google Scholar 

  2. Duran N, Rosa MA, D’Annibale A, Gianfreda L (2002) Enzyme Microb Technol 31:907

    Article  CAS  Google Scholar 

  3. Dalal S, Gupta MN (2007) Chemosphere 67:741

    Article  CAS  Google Scholar 

  4. Silva RA, Carmona-Ribeiro MA, Petri DFS (2007) Int J Biol Macromol 41:404

    Article  CAS  Google Scholar 

  5. Gomez JL, Bodalo A, Gomez E, Bastida J, Hidalgo MA, Gomez M (2006) Enzyme Microb Technol 39:1016

    Article  CAS  Google Scholar 

  6. Shukla SP, Modi K, Ghosh PK, Devi S (2004) J Appl Polym Sci 91:2063

    Article  CAS  Google Scholar 

  7. Pujari NS, Vaidya BK, Bagalkote S, Ponrathnam S, Nene S (2006) J Membrane Sci 285:395

    Article  CAS  Google Scholar 

  8. Li S, Hu J, Liu B (2004) Biosystems 77:25

    Article  CAS  Google Scholar 

  9. Rojas-Melgarejo F, Rodriguez-Lopez JN, Garcia-Canovas F, Garcia-Ruiz PA (2004) J Chem Technol Biotechnol 79:1148

    Article  CAS  Google Scholar 

  10. Agostinelli E, Belli F, Tempera G, Mura A, Floris G, Toniolo L, Vavasori A, Fabris S, Momo F, Stevanato R (2007) J Biotechnol 127:670

    Article  CAS  Google Scholar 

  11. Bindhu LV, Abraham ET (2003) J Appl Polym Sci 88:1456

    Article  CAS  Google Scholar 

  12. Oliveira GB, Filho JLL, Chaves MECC, Azevedo WM, Carvalho LB Jr (2008) React Funct Polym 68:27

    Article  CAS  Google Scholar 

  13. Sakurada I, Ikada Y, Kawahara T (1973) J Polym Sci 11:2329

    CAS  Google Scholar 

  14. Arslan M, Yiğitoğlu M, Şanlı O, Ünal Hİ (2003) Polym Bull 51:237

    Article  CAS  Google Scholar 

  15. Yiğitoğlu M, Arslan M (2007) Polym Bull 58:785

    Article  Google Scholar 

  16. Ünal Hİ, Coşkun R, Şanlı O, Yiğitoğlu M (1997) J Appl Polym Sci 64:1437

    Article  Google Scholar 

  17. Yiğitoğlu M, Arslan M (2005) Polym Bull 55:259

    Article  Google Scholar 

  18. Bağ H, Türker AR, Coşkun R, Saçak M, Yiğitoğlu M (2000) Spectrochim Acta Part B 55:1101

    Article  Google Scholar 

  19. Coşkun R, Yiğitoğlu M, Saçak M (2000) J Appl Polym Sci 75:766

    Article  Google Scholar 

  20. Yiğitoğlu M, Ersöz M, Coşkun R, Şanlı O, Ünal Hİ (1998) J Appl Polym Sci 68:1935

    Article  Google Scholar 

  21. Yiğitoğlu M, Arslan M (2007) E-Polymers 055

  22. Arslan M, Yiğitoğlu M (2008) J Appl Polym Sci 107:2846

    Article  CAS  Google Scholar 

  23. Arslan M, Yiğitoğlu M (2008) E-Polymers 016

  24. Temoçin Z, Yiğitoğlu M (2007) E-Polymers 070

  25. Vojinović V, Carvalho RH, Lemos F, Cabral JMS, Fonseca LP, Ferreira BS (2007) Biochem Eng J 35:126

    Article  Google Scholar 

  26. Kamal JKA, Behere DV (2008) Biochem Eng J 38:110

    Article  CAS  Google Scholar 

  27. Bryjak J (2003) Biochem Eng J 16:347

    Article  CAS  Google Scholar 

  28. Coşkun R, Soykan C (2006) J Polym Res 13:1

    Article  Google Scholar 

  29. Betancor L, Lopez-Gallego F, Hidalgo A, Alonso-Morales N, Mateo C, Fernandez-Lafuente R, Guisan JM (2006) Enzyme Microb Technol 39:877

    Article  CAS  Google Scholar 

  30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265

    CAS  Google Scholar 

  31. Halpin B, Pressey R, Jen J, Mony N (1989) J Food Sci 54:644

    Article  CAS  Google Scholar 

  32. Fernandes KF, Lima CS, Pinho H, Collins CH (2003) Proc Biochem 38:1379

    Article  CAS  Google Scholar 

  33. Atia KS, Ismail SA, Dessouki AM (2003) J Chem Technol Biotechnol 78:891

    Article  CAS  Google Scholar 

  34. Shukla SP, Devi S (2005) Proc Biochem 40:147

    Article  CAS  Google Scholar 

  35. Fernandes KF, Lima CS, Lopes FM, Collins CH (2004) Proc Biochem 39:957

    Article  CAS  Google Scholar 

  36. Chang MY, Juang RS (2005) Enzyme Microb Technol 36:75

    Article  CAS  Google Scholar 

  37. Paula AV, Urioste D, Santos JC, Castro HF (2007) J Chem Technol Biotechnol 82:281

    Article  CAS  Google Scholar 

  38. Caramori SS, Fernandes KF (2004) Proc Biochem 39:883

    Article  CAS  Google Scholar 

  39. Fatima A, Husain Q, Khan RH (2007) J Mol Catal B Enzym 47:66

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to the Kırıkkale University Research Fund for the financial support for this work.

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Arts, Kirikkale University, 71450, Kirikkale, Turkey

    Zülfikar Temoçin & Mustafa Yiğitoğlu

Authors
  1. Zülfikar Temoçin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mustafa Yiğitoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mustafa Yiğitoğlu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Temoçin, Z., Yiğitoğlu, M. Studies on the activity and stability of immobilized horseradish peroxidase on poly(ethylene terephthalate) grafted acrylamide fiber. Bioprocess Biosyst Eng 32, 467–474 (2009). https://doi.org/10.1007/s00449-008-0266-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-008-0266-9

Keywords

Search

Navigation