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Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts

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Abstract

Production of recombinant versatile peroxidase in Aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. To further this purpose, the cDNA encoding a versatile peroxidase of Pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alcA) promoter of Aspergillus nidulans. A dependence of recombinant peroxidase production on cultivation temperature was found. Lowering the culture temperature from 28 to 19 °C enhanced the level of active peroxidase 5.8-fold and reduced the effective proteolytic activity twofold. Thus, a maximum peroxidase activity of 466 U L-1 was reached. The same optimization scheme was applied to a recombinant Aspergillus niger that bore the alcohol dehydrogenase regulator (alcR), enabling transformation with the peroxidase cDNA under the same alcA promoter. However, with this strain, the peroxidase activity was not improved, while the effective proteolytic activity was increased between 3- and 11-fold compared to that obtained with A. nidulans.

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Acknowledgments

The authors thank M. A. Peñalva (CIB, CSIC, Madrid) for providing the A. nidulans strain and the plasmid palcA, B. Felenbock (University of Orsay, Paris) for the recombinant A. niger strain containing the alcA regulator, and P. J. Punt (TNO Voeding, Zeist, The Netherlands) for the PN7 vector. This work was partially supported by the EU contract “Fungal metalloenzymes oxidizing aromatic compounds of industrial interest” (QLK3-99-590), the Comunidad de Madrid, and the Spanish Commission of Science and Technology (BIO98-610 and BIO99-908).

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Authors and Affiliations

  1. Department of Chemical Engineering, Institute of Technology, 15706, Santiago de Compostela, Spain

    G. M. Eibes, T. A. Lú-Chau, G. Feijoo & J. M. Lema

  2. Centro de Investigaciones Biológicas, CSIC, Ramiro de Maezu 9, 28040, Madrid, Spain

    F. J. Ruiz-Dueñas, M. J. Martínez & A. T. Martínez

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  1. G. M. Eibes
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  2. T. A. Lú-Chau
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  3. F. J. Ruiz-Dueñas
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  4. G. Feijoo
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  5. M. J. Martínez
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  6. A. T. Martínez
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  7. J. M. Lema
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Correspondence to T. A. Lú-Chau.

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Eibes, G.M., Lú-Chau, T.A., Ruiz-Dueñas, F.J. et al. Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts. Bioprocess Biosyst Eng 32, 129–134 (2009). https://doi.org/10.1007/s00449-008-0231-7

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  • DOI: https://doi.org/10.1007/s00449-008-0231-7

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