Abstract
The effects of culture medium formulations on the kinetics of infective juvenile (IJ) production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture, were studied at the cylindrical-bottle scale using six culture media containing agave juice from Agave spp. among other ingredients. The IJ production kinetics was well modelled through a re-parameterised 3-parameter Gompertz model with kinetic parameters: IJ-lag phase λ IJ (day), maximum IJ-stage production rate m max (day−1), and IJ-multiplication factor (C IJ/C IJ,0)max(−). The variation of λ IJ was not very important within fermentations (10.3–16.2 days); nonetheless, important effects were observed on m max (32.8–241.2 days−1) and (C IJ/C IJ,0)max (66(−) to 611.4(−)). Particularly, maximum values of m max and (C IJ/C IJ,0)max were obtained in medium A4 (0.276 l l−1 agave juice, 17 g l−1 yeast extract, 12 g l−1 dried egg yolk, 0.025 l l−1 corn oil). Also, the maximum IJ concentration (249,444 per ml) was achieved in A4-fermentations.
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Abbreviations
- C :
-
total nematode concentration (individuals ml−1)
- C IJ :
-
infective juvenile stage concentration (individuals ml−1)
- C IJ,0 :
-
initial infective juvenile stage concentration (individuals ml−1)
- C IJ,max :
-
maximum infective juvenile stage concentration (individuals ml−1)
- (C IJ/C IJ,0):
-
dimensionless infective juvenile stage concentration (−)
- (C IJ/C IJ,0)max :
-
infective juvenile stage multiplication factor (−)
- [d(C IJ/C IJ,0)/dt]max or m max :
-
maximum infective juvenile stage production rate (day−1)
- λ IJ :
-
infective juvenile stage lag time (day)
- λ IJ,min :
-
minimum infective juvenile stage lag time (day)
- λ IJ,max :
-
maximum infective juvenile stage lag time (day)
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Acknowledgments
The authors thank financial support of Fondos Mixtos-Consejo Nacional de Ciencia y Tecnología-Gobierno del Estado de Hidalgo, México (Grant 200201-9206), and PIFI P/CA-3 2006-14-04 “Consolidación del Cuerpo Académico de Biotecnología Agroalimentaria”. Many thanks to Ometeotl-AC, Tlaxcala, México, for the agave juice supply. Technical assistance of R. Sanjuan-Galindo and molecular identification of the symbiotic bacterium by the group of Dr. Wacher-Rodarte are deeply acknowledged.
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Chavarría-Hernández, N., Islas-López, MA., Maciel-Vergara, G. et al. Kinetics of infective juvenile production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture. Bioprocess Biosyst Eng 31, 419–426 (2008). https://doi.org/10.1007/s00449-007-0178-0
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DOI: https://doi.org/10.1007/s00449-007-0178-0