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Evidence of thermostable amylolytic activity from Rhizopus microsporus var. rhizopodiformis using wheat bran and corncob as alternative carbon source

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Abstract

Rhizopus microsporus var. rhizopodiformis produced high levels of α-amylase and glucoamylase under solid state fermentation, with several agricultural residues, such as wheat bran, cassava flour, sugar cane bagasse, rice straw, corncob and crushed corncob as carbon sources. These materials were humidified with distilled water, tap water, or saline solutions—Segato Rizzatti (SR), Khanna or Vogel. The best substrate for amylase production was wheat bran with SR saline solution (1:2 v/v). Amylolytic activity was still improved (14.3%) with a mixture of wheat bran, corncob, starch and SR saline solution (1:1:0.3:4.6 w/w/w/v). The optimized culture conditions were initial pH 5, at 45 °C during 6 days and relative humidity around 76%. The crude extract exhibited temperature and pH optima around 65 °C and 4–5, respectively. Amylase activity was fully stable for 1 h at temperatures up to 75 °C, and at pH values between 2.5 and 7.5.

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Acknowledgments

This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho de Desenvolvimento Científico e Tecnológico (CNPq). H.F.T.; J.A.J.; M.L.T.M.P. are Research Fellows of CNPq. S.C.P.N. was Master recipient CAPES and this work is part of her Master Dissertation. We thank Ricardo F. Alarcon and Maurício de Oliveira for technical assistance.

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Authors and Affiliations

  1. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirao Preto, SP, 14040-901, Brazil

    S. C. Peixoto-Nogueira, L. H. S. Guimarães, J. A. Jorge, H. F. Terenzi & M. L. T. M. Polizeli

  2. Instituto de Química de Araraquara, UNESP, Araraquara, SP, Brazil

    V. C. Sandrim

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  1. S. C. Peixoto-Nogueira
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  2. V. C. Sandrim
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  3. L. H. S. Guimarães
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  4. J. A. Jorge
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  5. H. F. Terenzi
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  6. M. L. T. M. Polizeli
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Correspondence to M. L. T. M. Polizeli.

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Peixoto-Nogueira, S.C., Sandrim, V.C., Guimarães, L.H.S. et al. Evidence of thermostable amylolytic activity from Rhizopus microsporus var. rhizopodiformis using wheat bran and corncob as alternative carbon source. Bioprocess Biosyst Eng 31, 329–334 (2008). https://doi.org/10.1007/s00449-007-0166-4

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  • DOI: https://doi.org/10.1007/s00449-007-0166-4

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