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Optimization of physicochemical parameters for the enhancement of carbonyl reductase production by Candida viswanathii

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Abstract

Culture conditions have been optimized for a newly isolated yeast strain Candida viswanathii PBR2 which is capable of reducing a wide variety of aryl ketones with high stereospecificity. Studies on the culture conditions and catalytic performance of this microorganism showed that the carbonyl reductase occurs constitutively in the cells and its production is enhanced by feeding with acetophenone (2 mM) during the early period of cultivation. Mannitol (1%, wv−1) was found to be beneficial both for growth and enzyme production. Supplementation of the media with yeast extract (1.0%, wv−1) and Ca2+ (4 mM) enhanced the enzyme production. The optimal temperature and pH for the growth and enzyme production were 25 °C and 9.0, respectively. Excellent conversions along with almost absolute enantioselectivity were observed when the resting cells of this yeast strain were exploited to carry out the stereoselective reduction of a number of aryl ketones.

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Acknowledgements

One of the authors (PS) gratefully acknowledges Council of Scientific and Industrial Research, India for providing senior research fellowship. Gandhan S. Prasad is thankful to Department of Biotechnology (DBT), Government of India for the financial support. This is NIPER communication number 372.

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Authors and Affiliations

  1. Biocatalysis Laboratory, Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, 160 062, India

    Pankaj Soni & Uttam C. Banerjee

  2. Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Sector 39 A, Chandigarh, 160 016, India

    Gandhan S. Prasad

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  1. Pankaj Soni
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  2. Gandhan S. Prasad
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  3. Uttam C. Banerjee
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Correspondence to Uttam C. Banerjee.

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Soni, P., Prasad, G.S. & Banerjee, U.C. Optimization of physicochemical parameters for the enhancement of carbonyl reductase production by Candida viswanathii . Bioprocess Biosyst Eng 29, 149–156 (2006). https://doi.org/10.1007/s00449-006-0066-z

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