Abstract
Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions performed with palmitic acid by liquid-state and microwave esterification gave a degree of substitution (DS) of 62.08% (DS 1.45) and 42.06% (DS 0.98), respectively. Thermogravimetric analysis showed that onset of decomposition is at a higher temperature (above 600°C) for modified starch than the unmodified starch (280°C). Modified starch showed reduction in α-amylase digestibility compared to native starch (76.5–18%). Swelling power lowered for modified starch as esterification renders starch more hydrophobic, making it suitable for biomedical applications as materials for bone fixation and replacements, carriers for controlled release of drugs and bioactive agents. Thus enzymatic esterification is ecofriendly.
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References
Doane WM (1992) USDA research on starch-based biodegradable plastics. Starch 44:293–295
Shogren RL, Fanta GF, Doane WM (1994) Development of starch based plastics—a re examination of selected polymer systems in historical perspective. Starch 45:276–280
Roper H (1996) Application of starch and its derivatives. Carbohydr Eur 15:22–30
Aburto J, Alric I, Thiebaud S, Borredon E, Bikiaris D, Prinos J, Panayiotou C (1999) Synthesis, characterization, and biodegradability of fatty-acid esters of amylose and starch. J Appl Polym Sci 74:1440–1451
Aburto J, Thiebaud S, Alric I, Borredon E, Bikiaris D, Prinos J, Panayiotou C (1997) Properties of octanoated starch and its blends with polyethylene. Carbohydr Polym 34:101–112
Fang JM, Fowler PA, Tomkinson J, Hill CAS (2002) The preparation and characterisation of a series of chemically modified potato starches. Carbohydr Polym 47:245–252
Thiebaud S, Aburto J, Alric I, Borredon E, Bikiaris D, Prinos J, Panayiotou C (1997) Properties of fatty-acid esters of starch and their blends with LDPE. J Appl Polym Sci 65:705–721
Caddick S (1995) Microwave assisted organic reactions. Tetrahedron 51:10403–10432
Lewandowicz G, Fornal J, Walkowski A (1997) Effect of microwave radiation on potato tapioca starches. Carbohydr Polym 34:213
Loupy A, Pigeon P, Ramdani M, Jaquault P (1994) Solid–liquid phase transfer catalysis without solvent coupled with microwave irradiation: a quick and efficient method for saponification of esters. Synth Commun 24:159–165
Sowmya S, Balasubramanian KK (1994) Microwave induced ferrier rearrangement. Synth Commun 24:2097–2101
Noomrio MH, Khan MY, Dahot MU (1990) Purification and characterization of Cajanus cajan L. seed lipase. Proc Natl Chem Conf II:139–142
Miladinov VD, Hanna MA (1999) Physical and molecular properties of starch acetates extruded with water and ethanol. Ind Eng Chem Res 10:3892–3897
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426
Schoch TJ (1964) Swelling power and solubility of granular starches. In: Whistler RL (ed) Methods in carbohydrate chemistry, vol. 4. Academic, New York, pp 106–108
Sagar AD, Merrill EW (1995) Properties of fatty-acid esters of starch. J Appl Polym Sci 58:1647–1656
Goheen SM, Wool RP (1991) Degradation of polyethylene starch blends in soil. J Appl Polym Sci 42:2691–2701
Kacurakova M, Belton PS, Wilson RH, Hirsch J, Ebringerova A (1998) Hydration properties of xylan-type structure: an FTIR study of xylooligosaccharides. J Sci Food Agric 77:38–44
Kacurakova M, Wilson RH (2001) Developments in mid-infrared FT-IR spectroscopy of selected carbohydrates. Carbohydr Polym 44:291–303
Marcazzan M, Vianello F, Scarpa M, Rigo A (1999) An ESR assay for alpha-amylase activity toward succinylated starch, amylose and amylopectin. J Biochem Biophys Methods 38:191–202
Mack EJ, Okano T, Kim SW (1988) In: Peppas NA (ed) Hydrogels in medicine and pharmacy-polymers, vol. II. CRC, Boca Raton, p 65
Xua YX, Dzenis Y, Hannaa MA (2005) Water solubility, thermal characteristics and biodegradability of extruded starch acetate foams. Ind Crops Prod 21:361–368
Gomes ME, Ribeiro AS, Malafaya PB, Reis RL, Cunha AM (2001) A new approach based on injection moulding to produce biodegradable starch-based polymeric scaffolds: morphology, mechanical and degradation behaviour. Biomaterials 22(9):883–889
Reis RL, Cunha AM (2000) New degradable load-bearing biomaterials composed of reinforced starch based blends. J Appl Med Polym 4:1–5
Sousa RA, Mano JF, Reis RL, Cunha AM, Bevis MJ (2002) Mechanical performance of starch based bioactive composites moulded with preferred orientation for potential medical applications. Polym Eng Sci 42:1032–1045
Malafaya PB, Elvira C, Gallardo A, San Roman J, Reis RL (2001) Porous starch-based drug delivery systems processed by a microwave route. J Biomater Sci Polym Ed 12(11):1227–1241
Espigares I, Elvira C, Mano JF, Vasquez B, San Yoman J, Reis RL (2002) New partially degradable and bioactive acrylic bone cements based on starch blends and ceramic fillers. Biomaterials 23:1883–1895
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We acknowledge Director, RRL, for providing the necessary facilities to carry out the work.
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Rajan, A., Abraham, T.E. Enzymatic modification of cassava starch by bacterial lipase. Bioprocess Biosyst Eng 29, 65–71 (2006). https://doi.org/10.1007/s00449-006-0060-5
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DOI: https://doi.org/10.1007/s00449-006-0060-5