Abstract
In this paper we propose a methodology to determine the structure of the pseudo-stoichiometric coefficient matrix K in a mass balance-based model and to identify its coefficients from a set of available data. The first stage consists in estimating the number of reactions that must be taken into account to represent the main mass transfer within the bioreactor. This provides the dimension of K. Then we propose a method to directly determine the structure of the matrix (i.e. mainly its zeros and the signs of its coefficients). These methods are illustrated with simulations of a process of lipase production from olive oil by Candida rugosa.
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Acknowledgements
This work has been carried out with the support provided by the European commission, Information Society Technologies programme, Key action I Systems & Services for the Citizen, contract TELEMAC number IST-2000-28256. It also presents research results of the Belgian Programme on Inter-University Poles of Attraction initiated by the Belgian State, Prime Minister’s office for Science, Technology and Culture. The scientific responsibility rests with its authors.
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Bernard, O., Bastin, G. Identification of reaction networks for bioprocesses: determination of a partially unknown pseudo-stoichiometric matrix. Bioprocess Biosyst Eng 27, 293–301 (2005). https://doi.org/10.1007/s00449-005-0407-3
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DOI: https://doi.org/10.1007/s00449-005-0407-3