Bioprocess and Biosystems Engineering

, Volume 27, Issue 4, pp 249–254 | Cite as

Performance and Microbial Structure of a Combined Biofilm Reactor

  • Haiyan GuoEmail author
  • Jiti Zhou
  • Wang Jing
  • Xiuhong Zhang
  • Zhiyong Zhang
  • M. Salah Uddin
Original papers


A novel combined biofilm reactor was established and applied as a single treatment unit for carbon and nitrogen removal of wastewater. The nitrogen removal performance of the reactor at different levels of organic carbon (COD) loading was investigated when the influent total nitrogen (TN) loading was 0.74 g TN/m2 day. Continuous experimental results demonstrated that 80% nitrogen was eliminated when the influent COD loading ranged between 2.06 g and 3.92 g COD/m2 day. Microbial composition in the reactor was analyzed using fluorescent in situ hybridization (FISH) and conventional batch tests. The relative abundance of ammonia-oxidizing bacteria in the aerobic zone of the reactor measured by FISH was consistent with the result from conventional batch tests.


Combined biofilm reactor Wastewater treatment Nitrification Denitrification 


  1. 1.
    Del Pozo R, Diez V (2003) Organic matter removal in combined anaerobic—aerobic fixed-biofilm bioreactors. Water Res 37:3561–3568CrossRefGoogle Scholar
  2. 2.
    ten Have PJW, Willers HC, Derikx PJI (1994) Nitrification and denitrification in an activated sludge system for supernatant from settled sow manure with molasses as an extra carbon source. Bioresource Technol 47:135–141Google Scholar
  3. 3.
    Menoud P, Wong CH, Robinson HA, Farquhar A, Barford JP, Barton GW (1999) Simultaneous nitrification and denitrification using SIPORAX packing. Wat Sci Tech 40:153–160Google Scholar
  4. 4.
    Priyali Sen, Dentel SK (1998) Simultaneous nitrification-denitrification in a fluidized bed reactor. Wat Sci Tech 38:247–254Google Scholar
  5. 5.
    Tujiwara T, Somiya I, Tsuno H, Okuno Y (1998) Effect of draft tube diameter on nitrogen removal from domestic sewage in a draft tube type reactor. Wat Sci Tech 38:319–326Google Scholar
  6. 6.
    van Loosdrecht MCM (2000) Integration of nitrification and denitrification in biofilm airlift suspension reactors. Wat Sci Tech 41:97–103Google Scholar
  7. 7.
    Hsieh Y-L, Tseng S-K, Chang Y-J (2003) Nitrogen removal from wastewater using a double–biofilm reactor with a continuous-flow method. Bioresource Technology 88:107–113CrossRefGoogle Scholar
  8. 8.
    Hibiya K, Terada A, Tsuneda S, Hirata A (2003) Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane aerated biofilm reactor. J Biotechnol 100:23–32CrossRefGoogle Scholar
  9. 9.
    Nakano HK, Iwasawa O, Lee IT-J, Matsumura M (2004) Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics. Bioprocess Biosyst Eng 26:141–145CrossRefGoogle Scholar
  10. 10.
    Manz W, Wagner M, Amann R, Schleifer K-H (1994) In situ characterization of the microbial consortia active in two wastewater treatment plants. Water Res 28:1715–1723CrossRefGoogle Scholar
  11. 11.
    Wagner M, Amann R, Lemmer H, Schleifer KH (1993) Probing activated sludge with oligonucleotides specific for Proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure. Appl Environ Microbiol 59:1520–1525Google Scholar
  12. 12.
    APHA (1989) Standard methods for the examination of water and wastewater, 17th ed. New York, American Public Health AssociationGoogle Scholar
  13. 13.
    You SJ, Hsu CL, Chuang SH, Ouyang CF (2003) Nitrification efficiency and nitrifying bacteria aboundance in combined AS-RBC and A2O systems. Wat Res 37:2281–2290CrossRefGoogle Scholar
  14. 14.
    van Loosdrecht MCM, Tijhuis L, Wijdieks, Heijnen JJ (1998) Population distribution in aerobic biofilms on small suspended particles. Wat Sci Tech 31:163–171Google Scholar
  15. 15.
    Sakai Y, Miama T, Takahashi F (1997) Simutaneous removal of organic and nitrogen compounds in intermittently aerated activated sludge process using magnetic separation. Wat Res 31:2113–2116CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Haiyan Guo
    • 1
    Email author
  • Jiti Zhou
    • 1
  • Wang Jing
    • 1
  • Xiuhong Zhang
    • 1
  • Zhiyong Zhang
    • 1
  • M. Salah Uddin
    • 1
  1. 1.Department of Environmental Science and TechnologyDalian University of TechnologyDalianChina

Personalised recommendations