Bioprocess and Biosystems Engineering

, Volume 27, Issue 4, pp 215–222 | Cite as

Energy-efficient recovery of butanol from model solutions and fermentation broth by adsorption

  • N. QureshiEmail author
  • S. Hughes
  • I. S. Maddox
  • M. A. Cotta


This article discusses the separation of butanol from aqueous solutions and/or fermentation broth by adsorption. Butanol fermentation is also known as acetone butanol ethanol (ABE) or solvent fermentation. Adsorbents such as silicalite, resins (XAD-2, XAD-4, XAD-7, XAD-8, XAD-16), bone charcoal, activated charcoal, bonopore, and polyvinylpyridine have been studied. Use of silicalite appears to be the more attractive as it can be used to concentrate butanol from dilute solutions (5 to 790–810 g L−1) and results in complete desorption of butanol (or ABE). In addition, silicalite can be regenerated by heat treatment. The energy requirement for butanol recovery by adsorption–desorption processes has been calculated to be 1,948 kcal kg−1 butanol as compared to 5,789 kcal kg−1 butanol by steam stripping distillation. Other techniques such as gas stripping and pervaporation require 5,220 and 3,295 kcal kg−1 butanol, respectively.


Butanol Adsorption Silicalite ABE Recovery Polyvinylpyridine 



N. Qureshi would like to thank Holly Brining and Mark Maroon for their contribution to this manuscript.


  1. 1.
    Maddox IS (1989) The acetone-butanol-ethanol fermentation: recent progress in technology. Biotechnol Genet Eng Rev 7:189–220Google Scholar
  2. 2.
    Ladisch MR (1991) Fermentation-derived butanol and scenarios for its uses in energy-related applications. Enz Microbial Technol 13:280–283CrossRefGoogle Scholar
  3. 3.
    Ezeji TC, Qureshi N, Blaschek HP (2004) Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microbiol Biotechnol 63:653–658CrossRefGoogle Scholar
  4. 4.
    Qureshi N, Blaschek HP (2001) Evaluation of recent advances in butanol fermentation, upstream, and downstream processing. Bioprocess Biosystems Eng 24:219–226Google Scholar
  5. 5.
    Friedl A, Qureshi N, Maddox IS (1991) Continuous acetone-butanol-ethanol (ABE) fermentation using immobilized cells of Clostridium acetobutylicum in a packed bed reactor and integration with product removal by pervaporation. Biotechnol Bioeng 38:518–527CrossRefGoogle Scholar
  6. 6.
    Qureshi N, Maddox IS, Friedl A (1992) Application of continuous substrate feeding to the ABE fermentation: Relief of product inhibition using extraction, perstraction, stripping and pervaporation. Biotechnol Prog 8:382–390CrossRefGoogle Scholar
  7. 7.
    Qureshi N, Meagher MM, Huang J, Hutkins RW (2001) Acetone butanol ethanol (ABE) recovery by pervaporation using silicalite-silicone composite membrane from fed-batch reactor of Clostridium acetobutylicum. J Memb Sci 187:93–102CrossRefGoogle Scholar
  8. 8.
    Groot WJ, van der Lans RGJM, Luyben KChAM (1992) Technologies for butanol recovery integrated with fermentations. Process Biochem 27:61–75CrossRefGoogle Scholar
  9. 9.
    Qureshi N, Dien B, Saha BC, Ezeji TC, Blaschek HP, Cotta MA (2003) Separation of butanol from fermentation broth by pervaporation: mass flux and energy balance. In: Paper presented at the Annual Meeting of American Institute of Chemical Engineers (AIChE), San Francisco, CA, 162Google Scholar
  10. 10.
    Qureshi N, Cotta MA (2004) Energy requirement for butanol recovery from fermentation broth using adsorption as an alternative technique. In: Proceedings of 26th symposium on biotechnology for fuels and chemicals, Chattanooga, TN, p 116Google Scholar
  11. 11.
    Milestone NB, Bibby DM (1981) Concentration of alcohols by adsorption on silicalite. J Chem Technol Biotechnol 31:732–736Google Scholar
  12. 12.
    Das K, Soni BK, Chose, TK (1987) Static and column studies on selective adsorption-desorption of butanol. In: Proceedings 4th European congress on biotechnol 1:76–78Google Scholar
  13. 13.
    Meagher MM, Qureshi N, Hutkins RW (1998) Silicalite membrane and method for the selective recovery and concentration of acetone and butanol from model ABE solutions and fermentation broth. U.S. Patent 5,755,967Google Scholar
  14. 14.
    Groot WJ, Luyben KChAM (1986) In situ product recovery by adsorption in the butanol/isopropanol batch fermentation. Appl Microbiol Biotechnol 25:29–31CrossRefGoogle Scholar
  15. 15.
    Nielsen L, Larsson M, Holst O, Mattiasson B (1987) Polymers as adsorbents for extractive bioconversions applied to the ABE-fermentation. In: Proceedings 4th European congress on biotechnol 2:293Google Scholar
  16. 16.
    Nielsen L, Larsson M, Holst O, Mattiasson B (1988) Adsorbents for extractive bioconversion applied to the acetone-butanol fermentation. Appl Microbiol Biotechnol 28:335–339CrossRefGoogle Scholar
  17. 17.
    Yang X, Tsai G-J, Tsao GT (1994) Enhancement of in situ adsorption on the acetone-butanol fermentation by Clostridium acetobutylicum. Separation Technol 4:81–92Google Scholar
  18. 18.
    Maddox IS (1982) Use of silicalite for the adsorption of n-butanol from fermentation liquors. Biotechnol Lett 4:759–760Google Scholar
  19. 19.
    Ennis BM, Qureshi N, Maddox, IS (1987) In-line toxic product removal during solvent production by continuous fermentation using immobilized Clostridium acetobutylicum. Enzyme Microbial Technol 9:672–675Google Scholar
  20. 20.
    Yang X, Tsao GT (1995) Enhanced acetone-butanol fermentation using repeated fed-batch operation coupled with cell recycle by membrane and simultaneous removal of inhibitory products by adsorption. Biotechnol Bioeng 47:444–450Google Scholar
  21. 21.
    Holtzapple MT, Brown RF (1994) Conceptual design for a process to recover volatile solutes from aqueous solutions using silicalite. Separat Technol 4:213–229Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • N. Qureshi
    • 1
    Email author
  • S. Hughes
    • 2
  • I. S. Maddox
    • 3
  • M. A. Cotta
    • 1
  1. 1.United States Department of Agriculture, National Center for Agricultural Utilization Research (USDA, NCAUR)Fermentation BiotechnologyPeoriaUSA
  2. 2.USDA, NCAURBioproducts and BiocatalysisPeoriaUSA
  3. 3.Institute of Engineering and TechnologyMassey UniversityPalmerston NorthNew Zealand

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