Direct fermentation of potato starch wastewater to lactic acid by Rhizopus oryzae and Rhizopus arrhizus


The biochemical kinetic of direct fermentation for lactic acid production by fungal species of Rhizopus arrhizus 3,6017 and Rhizopus oryzae 2,062 was studied with respect to growth pH, temperature and substrate. The direct fermentation was characterized by starch hydrolysis, accumulation of reducing sugar, and production of lactic acid and fungal biomass. Starch hydrolysis, reducing sugar accumulation, biomass formation and lactic acid production were affected with the variations in pH, temperature, and starch source and concentration. A growth condition with starch concentration approximately 20 g/l at pH 6.0 and 30°C was favourable for both starch saccharification and lactic acid fermentation, resulting in lactic acid yield of 0.87–0.97 g/g starch associated with 1.5–2.0 g/l fungal biomass produced in 36 h fermentation. R. arrhizus 3,6017 had a higher capacity to produce lactic acid, while R. oryzae 2,062 produced more fungal biomass under similar conditions.

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This study was partly funded by an Early Career Research grant, The University of Queensland, Australia, SRF for ROCS, SEM of China and National Science Foundation of China (50276008). We would like to thank Freer Foods Pty Ltd, Brisbane, Australia for supplying starch stream samples.

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Correspondence to Li Ping Huang.

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Huang, L.P., Jin, B. & Lant, P. Direct fermentation of potato starch wastewater to lactic acid by Rhizopus oryzae and Rhizopus arrhizus . Bioprocess Biosyst Eng 27, 229–238 (2005).

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  • Fermentation
  • Lactic acid
  • Reducing sugar
  • Starch hydrolysis
  • Fungal biomass