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Effects of glucose limitation on biomass and spiramycin production by Streptomyces ambofaciens

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Abstract

Spiramycin production by Streptomyces ambofaciens Sp181110 with glucose as the carbon source was studied under a controlled nutritional environment. In a batch culture, the glucose excess after ammonium depletion led to pyruvate and α-ketoglutarate accumulation. 85 mg/l of spiramycin were produced in less than 70 h during the stationary and maintenance phase on these acids after glucose exhaustion. Fed-batch strategy was designed to study spiramycin production without by-product formation and glucose accumulation. In these conditions, up to 150 mg/l were produced in less than 80 h during the stationary phase on glucose. The antibiotic titre was found independent of the glucose feeding under carbon limitation and the importance of putative intracellular reserves formed after nutrient exhaustion was suggested. Besides, spiramycin production was not inhibited by the limiting flux of glucose.

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Acknowledgments

This study was supported by Sanofi-Aventis, Vitry sur Seine, France

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Authors and Affiliations

  1. Département de génie biologique et alimentaire, UMR-CNRS 5504 UR-INRA 792, Institut National des Sciences Appliquées, Complexe scientifique de Rangueil, 31077, Toulouse cedex, France

    V. Colombié, C. Bideaux, G. Goma & J. L. Uribelarrea

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  1. V. Colombié
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  2. C. Bideaux
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  3. G. Goma
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  4. J. L. Uribelarrea
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Correspondence to J. L. Uribelarrea.

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Colombié, V., Bideaux, C., Goma, G. et al. Effects of glucose limitation on biomass and spiramycin production by Streptomyces ambofaciens. Bioprocess Biosyst Eng 28, 55–61 (2005). https://doi.org/10.1007/s00449-005-0015-2

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  • DOI: https://doi.org/10.1007/s00449-005-0015-2

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