Skip to main content
SpringerLink
Log in

MIR spectroscopic analysis on sugar metabolic and ethanol productive kinetics of suspension TBY-2 and rice cells pre-cultured in various media

  • Original papers
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The influence of sugars in pre-cultivation media suspended plant cells on the kinetics of the sugar uptake and the ethanol production was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation with Nicotiana tabacum cv. Bright Yellow No.2 (TBY-2) cells and Oryza sativa L., Japonica, cv. Nipponbare (rice) cells, respectively, in pre-culture and culture media, which had various types of glucose, fructose, sucrose or glucose–fructose mixtures. The results confirmed the kinetic differences between the TBY-2 cells and rice cells. These results suggested that the TBY-2 cells consumed sugar before growth and the rice cells consumed sugar after growth, moreover, the ethanol content increased just after cell growth was activated based on the non-dimensional cultivation time for the cell growth behavior.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Mohr H, Schophfer P (1998) In: Amino S, Komamine A (eds) Plant physiology. Springer, Tokyo, pp 117–259

    Google Scholar 

  2. Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. The Plant cell (Suppl):185–205

    Google Scholar 

  3. Lalonde S, Boles E, Hellmann H, Barker L, Patrick JW, Frommer WB, Ward JM (1999) The dual function of sugar carriers: transport and sugar sensing. Plant Cell 11:707–26

    Article  CAS  Google Scholar 

  4. Harrick NJ (1967) Internal reflection spectroscopy. Review and supplement. Harrick Scientific Corporarion, New York

    Google Scholar 

  5. Mirabella FM, Harrick NJ (1985) Internal reflection spectroscopy. Review and supplement. Harrick Scientific Corporarion, New York

    Google Scholar 

  6. Bellon V, Vallat C, Goffinet D (1995) Quantitative analysis of individual sugars during starch hydorolysis by FT-IR/ATR spectroscopy. Part I: multivariate calibration study repeatability and reproducibility. Appl Spectrosc 49:556–562

    Google Scholar 

  7. McQueen DH, Wilson R, Kinnunen A, Jensen EP (1995) Comparison of two infrared spectroscopic methods for Cheese analysis. Talanta 42:2007–2015

    Article  CAS  Google Scholar 

  8. Kemsley EK, Holland JK, Defernez M, Wilson RH (1996) Detection of adulteration of Raspberry Purees using infrared spectroscopy and chemometrics. J Agric Food Chem 44:3864–3870

    Article  CAS  Google Scholar 

  9. Wilson RH (1990) Fourier transform mid-infrared spectroscopy for food analysis. Trends Anal Chem 9(4):127–131

    Article  CAS  Google Scholar 

  10. Van de Voort FR (1992) Fourier transform infrared spectroscopy applied to food analysis. Food Res Int 25:397–403

    CAS  Google Scholar 

  11. Kameoka T, Okuda T, Hashimoto A, Noro A, Shiinoki Y, Ito K (1998) FT-IR analysis of sugars in aqueous solutions using ATR method. J Jpn Soc Food Sci Technol 45:192–198

    CAS  Google Scholar 

  12. Kameoka T, Okuda T, Hashimoto A, Noro A, Shiinoki Y, Ito K (1998) A rapid FT-IR/ATR method for sugar content determination in food. J Jpn Soc Food Sci Technol 45:199–204

    CAS  Google Scholar 

  13. Kanou M, Nakanishi K, Hashimoto A, Kameoka T (2003) Infrared spectroscopic analysis of disaccharides in aqueous solutions. J Jpn Soc Food Sci Technol 50:57–62

    CAS  Google Scholar 

  14. Hashimoto A, Kameoka T (2000)Mid-infrared spectroscopic determination of sugar contents in plant-cell culture media using an ATR method. Appl Spectrosc 54:1005–1011

    Article  CAS  Google Scholar 

  15. Hashimoto A, Yamanaka A, Kanou M, Nahar F, Kameoka T (2003) Effect of pre-cultivation on sugar metabolic kinetics of suspension plant cells measured by mid-infrared spectroscopy. J Food Agric Environ 1(2):168–175

    CAS  Google Scholar 

  16. Wray V, Schiel O, Berlin J, Witte L (1985) Phosphorus-31 nuclear magnetic resonance investigation of the in vivo regulation of intracellular pH in cell suspension cultures of Nicotiana tabaccum: the effects of oxygen supply, nitrogen, and external pH change. Arch Biochem Biophys 236:731–740

    Article  CAS  Google Scholar 

  17. Hashimoto A, Yamanaka A, Kanou M, Nakanishi K, Kameoka T Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method. Bioprocess Biosyst Eng (in press)

  18. Hashimoto A, Nakanishi K, Motonaga Y, Kameoka T (2001) Sugar metabolic analysis of suspensions of plant cells using an FT-IR/ATR method. Biotechnol prog 17:560–564

    Article  CAS  Google Scholar 

  19. Hashimoto A, Nakanishi K, Yamanaka A, Nahar F, Motonaga Y, Kameoka T (2001) Mid-infrared spectroscopic analysis of sugar metabolism by rice cells suspending in culture medium. IFAC computer applications in biotechnology 2001. Elsevier Science Ltd., pp 379–384

  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tabacco tissue culture. Physiol Plant 15:473–497

    CAS  Google Scholar 

  21. Ohara K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14:1113–1121

    Google Scholar 

  22. Ohara K, Ojima K, Saigusa M, Fujiwara A (1975) Studies on the nutrition of rice cell culture II. Microelement requirement and the effects of deficiency. Plant Cell Physiol 16:73–81

    Google Scholar 

  23. Kyozuka J, Shimamoto K (1991) Transformation and regeneration of rice protoplasts. In: Lindsey K (ed) Plant tissue culture manual, B1. Kluwer, Dordrecht, pp 1–16

    Google Scholar 

  24. Cael J, Koening J, Blackwell J (1974) Infrared and Raman spectroscopy of carbohydrates, Part IV. Carbohydr Res 32:79–91

    Article  CAS  Google Scholar 

  25. Perata P, Matsukura C, Vernieri P, Yamaguchi J (1997) Sugar repression of a gibberellin-dependent signaling pathway in barley embryos. Plant Cell 9:2197–2208

    Article  CAS  Google Scholar 

  26. Morita A, Umemura T, Kuroyanagi M, Futsuhara K, Perata P, Yamaguchi J (1998) Functional dissection of a sugar-repressed Amylase gene (RAmyl A) promoter in rice embryos. FEBS Lett 423:81–85

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, 1515 Kamihama-cho, Tsu, Mie, 514-8507, Japan

    Atsushi Yamanaka, Atsushi Hashimoto, Mikihito Kanou & Takaharu Kameoka

Authors
  1. Atsushi Yamanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Atsushi Hashimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mikihito Kanou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takaharu Kameoka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atsushi Hashimoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamanaka, A., Hashimoto, A., Kanou, M. et al. MIR spectroscopic analysis on sugar metabolic and ethanol productive kinetics of suspension TBY-2 and rice cells pre-cultured in various media. Bioprocess Biosyst Eng 27, 125–133 (2005). https://doi.org/10.1007/s00449-004-0389-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-004-0389-6

Keywords

Search

Navigation