Skip to main content
SpringerLink
Log in

Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method

  • Original papers
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

A simple, rapid and accurate mid-infrared (MIR) spectroscopic method for simultaneously determining the product (ethanol) content and the nutrient (sugar) content in plant-cell culture media was developed using a Fourier transform infrared (FT-IR) spectrometer equipped with an attenuated total reflectance (ATR) accessory. We assessed the potential of this method by comparing it to a high-performance liquid chromatography (HPLC) method, and using the developed method to measure the ethanol and sugar contents simultaneously in liquid culture media with rice and tabacum cell suspensions, respectively. The experimental results suggest that the sugar consumption and ethanol production behaviors of the plant cell suspensions can be non-destructively and simultaneously monitored using the developed method. Furthermore, the spectroscopic method provided in this study could be developed into a technique that could be used to analyze the overall kinetics of the metabolism of the plant cell suspensions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Schmidt S, Kircher M, Kasala J, Locaj J (1998) Near infrared spectroscopy in fermentation and quality control for amino acid production. Bioprocess Eng 19:67–70

    Article  CAS  Google Scholar 

  2. Sivakesava S, Irudayaraj J, Ali D (2001) Simultaneous determination of multiple components in lactic acid fermentation using FT-MIR, NIR, and FT-Raman spectroscopic techniques. Process Biochem 37:371–378

    Article  CAS  Google Scholar 

  3. Pillonel L, Luginbühl W, Picque D, Schaller E, Tabacchi R, Boset JO (2002) Analytical methods for the determination of the geographic origin of Emmental cheese: mid- and near-infrared spectroscopy. Eur Food Res Technol 215:260–267

    CAS  Google Scholar 

  4. Chung H, Arnold MA, Rhiel M, Murhammer DW (1996) Simultaneous measurements of glucose, glutamine, ammonia, lactate, and glutaminate in aqueous solutions by near-infrared spectroscopy. Appl Spectrosc 50:270–276

    Article  CAS  Google Scholar 

  5. Hall JW, McNeil B, Rollins MJ, Draper I, Thompson BG, Macaloney G (1996) Near-infrared spectroscopic determination of acetate, ammonium, biomass, and glycerol in an industrial Escherichia coli fermentation. Appl Spectrosc 50:102–108

    Article  CAS  Google Scholar 

  6. Yano T, Suehara K, Nakano Y (1998) Determination of the content of water and rice bran in solid media used for mushroom cultivation using near-infrared spectroscopy. J Ferment Bioeng 86:472–476

    CAS  Google Scholar 

  7. Bernath PF (1995) Spectra of atoms and molecules. Oxford University Press, New York

    Google Scholar 

  8. Harrick NJ (1967) Internal reflection spectroscopy. Harrick Scientific Corporation, New York

    Google Scholar 

  9. Mirabella FM, Harrick NJ (1985) Internal reflection spectroscopy: review and supplement. Harrick Scientific Corporation, New York

    Google Scholar 

  10. Heise HM, Küpper L, Pittermann W, Butvina LN (2001) New tool for epidermal and cosmetic formulation studies by attenuated total-reflection spectroscopy using a flexible mid-infrared fiber probe. Fresen J Anal Chem 371:753–757

    CAS  Google Scholar 

  11. Maculey-Patrick S, Arnold SA, McCarthy B, Harvey LM, McNeil B (2003) Attenuated total reflectance fourier transform mid-infrared spectroscopic quantification of Sorbitor and Sorbose during a Gluconobacter biotransformation process. Biotechnol Lett 25:257–260

    Google Scholar 

  12. Pollard DJ, Buccino R, Connors NC, Kirschner TF, Olewinski RC, Saini K, Salmon PM (2001) Real-time analyte monitoring of a fungal fermentation, at Pilot scale, using situ mid-infrared spectroscopy. Bioprocess Biosyst Eng 24:13–24

    CAS  Google Scholar 

  13. Toraño JS, van Hattum SHC (2001) Quantitative analysis of active compounds in pharmaceutical preparations by use of attenuated total-reflection fourier transform mid-infrared spectrophotometry and the internal standard method. Fresen J Anal Chem 371:532–535

    Google Scholar 

  14. Bellon V, Vallat C, Goffinet D (1995) Quantitative analysis of individual sugars during starch hydrolysis by FT-IR/ATR spectroscopy. Part I: multivariate calibration study—repeatability and reproducibility. Appl Spectrosc 49:556–562

    Google Scholar 

  15. Dupuy N, Huvenne JP, Sombret B, Legrand P (1993) Quantitative analysis by mid-infrared spectrometry in food and agro-industrial fields. J Mol Struc 294:223–226

    Article  CAS  Google Scholar 

  16. Kemsley EK, Holland JK, Defernez M, Wilson RH (1996) Detection of adulteration of raspberry purees using infrared spectroscopy and chemometrics. J Agr Food Chem 44:3864–3870

    Article  CAS  Google Scholar 

  17. Schindler R, Lendl B (1999) Simultaneous determination of enzyme activities by FTIR-spectroscopy in an one-step assay. Anal Chim Acta 391:19–28

    Article  CAS  Google Scholar 

  18. Kameoka T, Okuda T, Hashimoto A, Noro A, Shiinoki Y, Ito K (1998) FT-IR analysis of sugars in aqueous solution using ATR method (in Japanese). J Jpn Soc Food Sci Technol 45:192–198

    CAS  Google Scholar 

  19. Kameoka T, Okuda T, Hashimoto A, Noro A, Shiinoki Y, Ito K (1998) A rapid FT-IR/ATR method for sugar content determination in food (in Japanese). J Jpn Soc Food Sci Technol 45:199–204

    CAS  Google Scholar 

  20. Hashimoto A, Kameoka T (2000) Mid infrared spectroscopic determination of sugar content in plant-cell culture media using an FT-IR/ATR method. Appl Spectrosc 54:1005–1011

    Article  CAS  Google Scholar 

  21. Hashimoto A, Nakanishi K, Motonaga Y, Kameoka T (2001) Sugar metabolic analysis of suspensions of plant cells using an FT-IR/ATR method. Biotechnol Prog 17:560–564

    Article  CAS  Google Scholar 

  22. Hashimoto A, Yamanaka A, Kanou M, Nahar F, Kameoka T (2003) Effects on pre-cultivation on sugar metabolic kinetics of suspension plant cells measured by mid-infrared spectroscopy. Food Agric Environ 1:168–175

    CAS  Google Scholar 

  23. Hashimoto A, Nakanishi K, Yamanaka A, Nahar F, Motonaga Y, Kameoka T (2002) Mid-infrared spectroscopic analysis of sugar metabolism by rice cells suspending in culture medium. In: Dochain D, Perrier M (eds) Computer applications in biotechnology 2001. Elsevier, Oxford, pp 379–384

    Google Scholar 

  24. Ohara K, Ojima K, Saigusa M, Fujiwara A (1975) Studies on the nutrition of rice cell culture II. Microelement requirement and the effects of deficiency. Plant Cell Physiol 16:73–81

    Google Scholar 

  25. Ohara K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14:1113–1121

    Google Scholar 

  26. Kyozuka J, Shimamoto K (1991) Transformation and regeneration of rice protoplasts In: Lindsey K (ed) Plant tissue culture manual, B1. Kluwer, Dordrecht, pp 1–16

    Google Scholar 

  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tabocco tissue culture. Physiol Plant 15:473–497

    CAS  Google Scholar 

  28. Cael J, Koening J, Blackwell J (1974) Infrared and Raman spectroscopy of carbohydrates. Part IV. Carbohyd Res 32:79–91

    CAS  Google Scholar 

  29. Nakanishi K, Hashimoto A, Kanou M, Pan T, Kameoka T (2003) Mid-infrared spectroscopic measurement of ionic dissociative materials in metabolic pathway. Appl Spectrosc 57:1510–1516

    Article  CAS  Google Scholar 

  30. Pan T, Hashimoto A, Kanou M, Nakanishi K, Kameoka T (2003) Development of a quantification system of ionic dissociative materials in the glycolytic pathway using an FT-IR/ATR method. Bioproc Biosyst Eng 26:133–139

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, 1515 Kamihama-cho, Tsu, Mie, 514-8507, Japan

    Atsushi Hashimoto, Atsushi Yamanaka, Mikihito Kanou, Kenichi Nakanishi & Takaharu Kameoka

Authors
  1. Atsushi Hashimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Atsushi Yamanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mikihito Kanou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kenichi Nakanishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takaharu Kameoka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atsushi Hashimoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hashimoto, A., Yamanaka, A., Kanou, M. et al. Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method. Bioprocess Biosyst Eng 27, 115–123 (2005). https://doi.org/10.1007/s00449-004-0388-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-004-0388-7

Keywords

Search

Navigation