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Bovine plasma protein fractionation by ion exchange chromatography

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Abstract

An ion exchange chromatography process was developed to separate the main protein fractions of bovine blood plasma using a composite material, Q-HyperD resin, and a gel material, DEAE-Sepharose. The experiments were carried out at semipreparative scale. It was necessary to establish analytical methods of electrophoresis and HPLC to identify the fractionated proteins. Results show that these materials are able to adequately fractionate different protein groups from the raw blood plasma. This method may be used to avoid chemical fractionation using agents such as ethanol or PEG and, thus, decrease protein denaturation of the different fractions to be used for research or pharmaceutical purposes. The Q-HyperD resin presents a better retention capacity for plasma protein than DEAE-Sepharose under the experimental conditions employed.

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Authors and Affiliations

  1. Project Management Area, University of Oviedo, C/ Independencia,13, Oviedo, 33003, Spain

    M. Rendueles

  2. Department of Chemical Engineering and Environmental Technology, University of Oviedo, C/ Julián Clavería 8, Oviedo, 33071, Spain

    F. Moure & M. Díaz

Authors
  1. F. Moure
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  2. M. Rendueles
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  3. M. Díaz
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Correspondence to M. Díaz.

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Moure, F., Rendueles, M. & Díaz, M. Bovine plasma protein fractionation by ion exchange chromatography. Bioprocess Biosyst Eng 27, 17–24 (2004). https://doi.org/10.1007/s00449-004-0372-2

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  • DOI: https://doi.org/10.1007/s00449-004-0372-2

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