Abstract
All orchids and pyroloids are mycoheterotrophic at least in the early stage. Many species are predisposed to mycoheterotrophic nutrition even in the adult stage, due to the initial mycoheterotrophy during germination. Although other green plants, such as gentian species, also produce numerous minute seeds, whose germination may depend on fungal associations to meet C demands, physiological evidence for partial mycoheterotrophy in the adult stage is lacking for most candidate taxa. Here, we compared the natural abundances of 13C and 15N isotopes in the AM-associated gentian species Pterygocalyx volubilis growing in high-light-intensity habitats with those of co-occurring autotrophic C3 and C4 plants and AM fungal spores. We found that P. volubilis was significantly enriched in 13C compared with the surrounding C3 plants, which suggests the transfer of some C from the surrounding autotrophic plants through shared AM networks. In addition, the intermediate δ15N values of P. volubilis, between those of autotrophic plants and AM fungal spores, provide further evidence for partial mycoheterotrophy in P. volubilis. Although it is often considered that light deficiency selects partial mycoheterotrophy, we show that partial mycoheterotrophy in AM-forming plants can evolve even under light-saturated conditions. The fact that there have been relatively few descriptions of partial mycoheterotrophy in AM plants may not necessarily reflect the rarity of such associations. In conclusion, partial mycoheterotrophy in AM plants may be more common than hitherto believed.
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Acknowledgements
We thank Mr. Masayuki Sato for help with the field study. We also thank Dr. Naohiko Ohkouchi and Dr. Hidetaka Nomaki for helping us to perform microscale isotope analysis. We also thank Dr. Masanori Saito for advice on the identification of AM fungal spores.
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This work was financially supported by the JSPS KAKENHI Grant nos. 17H05016 (KS), 17J04991 (JM), and 18K19356 (HT).
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KS planned and designed the research, collected the materials, carried out laboratory work and analyses, and wrote the initial draft. MJ, NOO, and HT conducted laboratory work and carried out analyses. SM and RS conducted the laboratory work. All authors contributed to the manuscript.
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Communicated by Susana Rodriguez Echeverria.
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Suetsugu, K., Matsubayashi, J., Ogawa, N.O. et al. Isotopic evidence of arbuscular mycorrhizal cheating in a grassland gentian species. Oecologia 192, 929–937 (2020). https://doi.org/10.1007/s00442-020-04631-x
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DOI: https://doi.org/10.1007/s00442-020-04631-x