Oecologia

, Volume 185, Issue 2, pp 195–203 | Cite as

Chronic stress, energy transduction, and free-radical production in a reptile

  • Yann Voituron
  • Rémy Josserand
  • Jean-François Le Galliard
  • Claudy Haussy
  • Damien Roussel
  • Caroline Romestaing
  • Sandrine Meylan
Physiological ecology - original research

Abstract

Stress hormones, such as corticosterone, play a crucial role in orchestrating physiological reaction patterns shaping adapted responses to stressful environments. Concepts aiming at predicting individual and population responses to environmental stress typically consider that stress hormones and their effects on metabolic rate provide appropriate proxies for the energy budget. However, uncoupling between the biochemical processes of respiration, ATP production, and free-radical production in mitochondria may play a fundamental role in the stress response and associated life histories. In this study, we aim at dissecting sub-cellular mechanisms that link these three processes by investigating both whole-organism metabolism, liver mitochondrial oxidative phosphorylation processes (O2 consumption and ATP production) and ROS emission in Zootoca vivipara individuals exposed 21 days to corticosterone relative to a placebo. Corticosterone enhancement had no effect on mitochondrial activity and efficiency. In parallel, the corticosterone treatment increased liver mass and mitochondrial protein content suggesting a higher liver ATP production. We also found a negative correlation between mitochondrial ROS emission and plasma corticosterone level. These results provide a proximal explanation for enhanced survival after chronic exposure to corticosterone in this species. Importantly, none of these modifications affected resting whole-body metabolic rate. Oxygen consumption, ATP, and ROS emission were thus independently affected in responses to corticosterone increase suggesting that concepts and models aiming at linking environmental stress and individual responses may misestimate energy allocation possibilities.

Keywords

Corticosterone Reptile Mitochondrial efficiency Allostatic overload ROS emission and ATP production Oxygen consumption 

Supplementary material

442_2017_3933_MOESM1_ESM.pptx (258 kb)
Supplementary material 1 (PPTX 257 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Laboratoire d’Ecologie des Hydrosystèmes Naturels et Anthropisés (U.M.R. CNRS 5023)Université Claude Bernard Lyon1, Université de LyonVilleurbanne CedexFrance
  2. 2.Institut d’Ecologie et des Sciences, de l’Environnement de Paris (iEES Paris)-UPMC-CNRSParis Cedex 05France
  3. 3.Ecole Normale SupérieurePSL Research University, CNRS, Centre de recherche en écologie expérimentale et prédictive (CEREEP-Ecotron IleDeFrance), UMS 3194Saint-Pierre-Lès-NemoursFrance
  4. 4.ESPE de Paris, Université Sorbonne Paris IVParisFrance

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