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Variability in bumblebee pollination buzzes affects the quantity of pollen released from flowers

Abstract

Buzz-pollination is a plant strategy that promotes gamete transfer by requiring a pollinator, typically bees (Hymenoptera: Apoidea), to vibrate a flower’s anthers in order to extract pollen. Although buzz-pollination is widespread in angiosperms with over 20,000 species using it, little is known about the functional connection between natural variation in buzzing vibrations and the amount of pollen that can be extracted from anthers. We characterized variability in the vibrations produced by Bombus terrestris bumblebees while collecting pollen from Solanum rostratum (Solanaceae), a buzz-pollinated plant. We found substantial variation in several buzzing properties both within and among workers from a single colony. As expected, some of this variation was predicted by the physical attributes of individual bumblebees: heavier workers produced buzzes of greater amplitude. We then constructed artificial “pollination buzzes” that varied in three parameters (peak frequency, peak amplitude, and duration), and stimulated S. rostratum flowers with these synthetic buzzes to quantify the relationship between buzz properties and pollen removal. We found that greater amplitude and longer duration buzzes ejected substantially more pollen, while frequency had no directional effect and only a weak quadratic effect on the amount of pollen removed. These findings suggest that foraging bumblebees may improve pollen collection by increasing the duration or amplitude of their buzzes. Moreover, given that amplitude is positively correlated with mass, preferential foraging by heavier workers is likely to result in the largest pollen yields per bee, and this could have significant consequences for the success of a colony foraging on buzz-pollinated flowers.

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Acknowledgments

We thank D. Boles for providing assistance with laser recordings, plant care, and bumblebee maintenance, and G. McLeod for SEM assistance. This study was funded in part by a Royal Society of Edinburgh International Travel Grant to L.F.B. and P.A.D., a Carnegie Trust Undergraduate Vacation Scholarship to D. Boles, a BES (University of Stirling) Undergraduate Vacation Scholarship to D.S.V. and a Royal Society of London Research Grant (RG2010R1) to M.V.M. This manuscript was improved by comments provided by two anonymous reviewers. The experiments conform to the current laws of the University of Stirling and the UK.

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Correspondence to Paul A. De Luca.

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Communicated by Diethart Matthies.

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De Luca, P.A., Bussière, L.F., Souto-Vilaros, D. et al. Variability in bumblebee pollination buzzes affects the quantity of pollen released from flowers. Oecologia 172, 805–816 (2013). https://doi.org/10.1007/s00442-012-2535-1

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  • DOI: https://doi.org/10.1007/s00442-012-2535-1

Keywords

  • Bombus terrestris
  • Solanum rostratum
  • Pollinator mass
  • Poricidal anthers
  • Buzz-pollination