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Rhizosphere interactions, carbon allocation, and nitrogen acquisition of two perennial North American grasses in response to defoliation and elevated atmospheric CO2

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Abstract

Carbon allocation and N acquisition by plants following defoliation may be linked through plant–microbe interactions in the rhizosphere. Plant C allocation patterns and rhizosphere interactions can also be affected by rising atmospheric CO2 concentrations, which in turn could influence plant and microbial responses to defoliation. We studied two widespread perennial grasses native to rangelands of western North America to test whether (1) defoliation-induced enhancement of rhizodeposition would stimulate rhizosphere N availability and plant N uptake, and (2) defoliation-induced enhancement of rhizodeposition, and associated effects on soil N availability, would increase under elevated CO2. Both species were grown at ambient (400 μL L−1) and elevated (780 μL L−1) atmospheric [CO2] under water-limiting conditions. Plant, soil and microbial responses were measured 1 and 8 days after a defoliation treatment. Contrary to our hypotheses, we found that defoliation and elevated CO2 both reduced carbon inputs to the rhizosphere of Bouteloua gracilis (C4) and Pascopyrum smithii (C3). However, both species also increased N allocation to shoots of defoliated versus non-defoliated plants 8 days after treatment. This response was greatest for P. smithii, and was associated with negative defoliation effects on root biomass and N content and reduced allocation of post-defoliation assimilate to roots. In contrast, B. gracilis increased allocation of post-defoliation assimilate to roots, and did not exhibit defoliation-induced reductions in root biomass or N content. Our findings highlight key differences between these species in how post-defoliation C allocation to roots versus shoots is linked to shoot N yield, but indicate that defoliation-induced enhancement of shoot N concentration and N yield is not mediated by increased C allocation to the rhizosphere.

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Acknowledgments

We thank Dan LeCain for assistance with leaf water potential measurements, experimental set-up and greenhouse maintenance, and Caitlin Brooks, Ally Eden, Erik Hardy, Pat McCusker and David Smith for assistance with plant harvests and laboratory analyses. We thank Daniel Milchunas, Shenglei Fu, Christian Korner, and three anonymous reviewers for thoughtful reviews that improved the manuscript.

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Authors and Affiliations

  1. Agricultural Research Service, Rangeland Resources Research Unit, USDA, 1701 Centre Ave, Fort Collins, CO, 80526, USA

    David J. Augustine, Feike A. Dijkstra & Jack A. Morgan

  2. Department of Biology, Washington and Lee University, Lexington, VA, 24450, USA

    E. William Hamilton III

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  1. David J. Augustine
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  2. Feike A. Dijkstra
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  3. E. William Hamilton III
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  4. Jack A. Morgan
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Correspondence to David J. Augustine.

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Communicated by Christian Körner.

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Augustine, D.J., Dijkstra, F.A., William Hamilton III, E. et al. Rhizosphere interactions, carbon allocation, and nitrogen acquisition of two perennial North American grasses in response to defoliation and elevated atmospheric CO2 . Oecologia 165, 755–770 (2011). https://doi.org/10.1007/s00442-010-1845-4

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