Seasonally dry tropical forests (SDTF) are characterized by pronounced seasonality in rainfall, and as a result trees in these forests must endure seasonal variation in soil water availability. Furthermore, SDTF on the northern Yucatan Peninsula, Mexico, have a legacy of disturbances, thereby creating a patchy mosaic of different seral stages undergoing secondary succession. We examined the water status of six canopy tree species, representing contrasting leaf phenology (evergreen vs. drought-deciduous) at three seral stages along a fire chronosequence in order to better understand strategies that trees use to overcome seasonal water limitations. The early-seral forest was characterized by high soil water evaporation and low soil moisture, and consequently early-seral trees exhibited lower midday bulk leaf water potentials (ΨL) relative to late-seral trees (−1.01 ± 0.14 and −0.54 ± 0.07 MPa, respectively). Although ΨL did not differ between evergreen and drought-deciduous trees, results from stable isotope analyses indicated different strategies to overcome seasonal water limitations. Differences were especially pronounced in the early-seral stage where evergreen trees had significantly lower xylem water δ18O values relative to drought-deciduous trees (−2.6 ± 0.5 and 0.3 ± 0.6‰, respectively), indicating evergreen species used deeper sources of water. In contrast, drought-deciduous trees showed greater enrichment of foliar 18O (∆18Ol) and 13C, suggesting lower stomatal conductance and greater water-use efficiency. Thus, the rapid development of deep roots appears to be an important strategy enabling evergreen species to overcome seasonal water limitation, whereas, in addition to losing a portion of their leaves, drought-deciduous trees minimize water loss from remaining leaves during the dry season.
Seasonally dry tropical forests (SDTF) are widely distributed across the tropics and are characterized by pronounced seasonality in rainfall resulting in seasonal variation in soil water availability (Murphy and Lugo 1986; Mooney et al. 1995). These forests represent roughly 40% of the landmass covered by all tropical forests (Murphy and Lugo 1986) and cover an estimated area of 1.05 × 106 km2 (Miles et al. 2006). However, relatively little research has been done in SDTF compared to wet tropical or temperate forests. Because of exceptionally high rates of land-use change, SDTF are considered to be the most threatened of all major tropical forest types, with less than 10% of mature forest remaining in many areas (Murphy and Lugo 1986; Janzen 1988; Bullock et al. 1995). The primary uses of SDTF have been shifting towards agriculture, selective logging, tree plantations, and conversion to grasslands for grazing (Murphy and Lugo 1986). Furthermore, SDTF on the Yucatan Peninsula, Mexico, are subject to natural disturbances such as hurricanes and fire (Boose et al. 2003; Whigham et al. 2003). Consequently, SDTF on the Yucatan Peninsula are characterized by a patchy mosaic of different seral stages (or age classes) of forests undergoing secondary succession.
As a result of decreased soil water availability, a number of trees in SDTF lose a large portion of their leaves during the dry season (drought-deciduous) (Murphy and Lugo 1986). Previous studies demonstrated that deciduous species minimize water loss during rainless periods and therefore dominate drier forests along precipitation gradients in the lowland tropics (Murphy and Lugo 1986; Santiago et al. 2004). Numerous studies have also shown a high degree of vertical partitioning of soil water resources among tropical forest tree species (Jackson et al. 1995, 1999; Meinzer et al. 1999; Querejeta et al. 2007). The degree of soil water partitioning as well as variation in timing of leaf production has therefore been hypothesized to decrease competition and promote tree species coexistence in SDTF. However, the majority of these studies have focused on tree species in mature forests. Early- and late-seral trees experience different abiotic and biotic environments that can differentially alter resource acquisition, growth and survival (Brown and Lugo 1990; Allen et al. 2003a). Consequently, there is a critical need to better understand how trees in different seral stages overcome seasonal water limitations in SDTF.
The Yucatan Peninsula is a low-lying karst platform characterized by shallow soils that have limited water storage capacity (Weidie 1985; Weisbach et al. 2002). Trees must therefore endure 4–6 months of low soil water availability in the strongly seasonal dry climate on the northern Yucatan Peninsula. Despite these seasonal water limitations, some native trees maintain their foliage during the dry season, suggesting they have access to a more permanent water source, such as groundwater (Benjamin et al. 2001). Schenk and Jackson (2002) showed that rooting depth in the tropics is positively correlated with the length of the dry season, which suggests that deep rooting may be functionally important for trees in SDTF. At the El Eden Ecological Reserve, where this research was conducted, the permanent groundwater table is 4–5 m from the soil surface (Allen et al. 2003b). Other studies have shown tree species sending roots to water tables at similar or greater depths in a number of different ecosystems (Canadell et al. 1996 and references therein; Farrington et al. 1996; Zencich et al. 2002). It is therefore often assumed that trees, especially evergreen tree species on the northern Yucatan Peninsula, use groundwater to overcome water limitations during the dry season. However, in studies near Merida, Yucatan, where the water table is deeper (9–20 m depth), evergreen trees appeared to utilize water sources stored in the limestone bedrock and not groundwater (Querejeta et al. 2007).
Traditionally, the sources of water used by trees in tropical forests have been difficult to determine because of the large and intricate root biomass and high species diversity characteristic of these ecosystems. However, advances in stable isotope techniques have enabled researchers to more accurately determine water sources for individual plants (Ehleringer and Dawson 1992; Brunel et al. 1995). During water uptake and transport within woody tissue, the oxygen isotopic signature (δ18O) of plant xylem water remains unaltered, and therefore reflects the various zone(s) and depth(s) from which plants extract soil water (White et al. 1985; Dawson and Ehleringer 1991). Because of limited precipitation during the dry season, the upper soil layers become enriched in the heavier isotopes (18O) due to evaporative fractionation, thereby creating a vertical gradient in the oxygen isotopic signature with soil depth (Barnes and Turner 1998). The oxygen isotopic signature of xylem water can therefore be compared to the δ18O signature of water found throughout the soil profile to better understand the sources of water used by individual plants (Ehleringer and Dawson 1992; Jackson et al. 1995; Bonal et al. 2000; Querejeta et al. 2007).
Additionally, the stable isotopic composition of bulk leaf material can be used to infer photosynthetic gas exchange properties. The carbon isotopic composition of plant material (δ13C) is linearly related to the ratio between the partial pressure of CO2 in the leaf intercellular spaces and that of ambient air. Thus, foliar δ13C provides a useful index for assessing intrinsic water-use efficiency (WUE), which is the ratio of photosynthetic carbon fixation to stomatal conductance (Farquhar et al. 1982, 1989; see Dawson et al. 2002). The oxygen isotopic composition of plant organic material (δ18O), on the other hand, provides a time-integrated measure of stomatal conductance when other sources of variation (mainly source water δ18O) are minimized (Saurer et al. 1997; Barbour and Farquhar 2000; Barbour et al. 2000; Barbour 2007; Farquhar et al. 2007). The δ18O signature of plant tissue is determined by the integrated leaf-to-air vapor pressure gradient during transpiration (Farquhar et al. 1998) and is strongly influenced by the plant physiological response to changes in environmental conditions such as atmospheric humidity and soil moisture (Powers et al. 2009). Thus, the simultaneous measurements of δ13C and δ18O in leaf organic material can help separate the independent effects of carbon fixation and stomatal conductance on δ13C because both δ18O and δ13C are impacted by stomatal conductance, whereas δ18O is unaffected by photosynthetic rate (Keitel et al. 2003; Scheidegger et al. 2000; Grams et al. 2007; Sullivan and Welker 2007).
In this study, we examined the water status of six canopy tree species, representing contrasting leaf phenology (evergreen vs. drought-deciduous) at three seral stages along a fire chronosequence on the northern Yucatan Peninsula. We also present isotope data on plant xylem water (δ18O), foliar carbon (δ13C), and enrichment of foliar δ18O relative to source water (∆18Ol) in order to better understand strategies trees use to overcome water limitations during the dry season. We hypothesized that trees in the early-seral stage would exhibit lower bulk leaf water potentials as a result of using shallower water sources compared to trees in the late-seral stage that have access to deeper sources of water. We also hypothesized that evergreen trees would use deeper water sources from deeper soil layers relative to drought-deciduous trees, whereas drought-deciduous trees would have higher foliar ∆18Ol and δ13C values, indicating reduced stomatal conductance and subsequently greater WUE.
Materials and methods
The study was carried out at El Eden Ecological Reserve, a 2,500-ha seasonal tropical forest reserve located in the northeastern corner of the Yucatan Peninsula, Mexico (21°12.61′N, 87°10.93′W). The mean annual temperature of the site is 24.2°C. The region receives an average of 1,650 mm of annual precipitation with a wet season from May to December. The dry season is from January to April when precipitation is <100 mm per month. The soils at the Reserve are shallow (<20 cm) with ca 30% soil organic matter, pH 7.5, bulk density of 0.35 g/cm3, and overlay limestone bedrock (Allen et al. 2003a; Vargas and Allen 2008).
The vegetation of the Reserve is described in Schutlz (2005), and consists of a mosaic of seral stages undergoing different stages of secondary succession (Allen et al. 2003b; Hasselquist et al. 2010). For this study, we selected three different seral stages representing a chronosequence of secondary succession ranging in age from an area burned 9 years previously (early-seral stage), an area burned 18 years previously (mid-seral stage) to an area that had not been disturbed for more than 60 years (late-seral stage). All seral stages of the chronosequence were located within a distance of 5 km. Greater detail on the site characteristics can be found in Vargas et al. (2008).
Plant and soil/bedrock samples were collected simultaneously within each seral stage in April 2007. We selected six tree species representing contrasting leaf phenology (evergreen vs. drought-deciduous) for isotopic analyses (Table 1). These species were chosen because they are abundant across the site and are found throughout the chronosequence. The reason that all three drought-deciduous species were from a single family is because the majority of the drought-deciduous species at our site belong to the Fabaceae family. The limitation to this sampling scheme is that physiological variation between species with evergreen versus drought-deciduous phenology could be the result of divergences deep in the phylogenetic tree. However, this would largely reflect the variation of phenological strategies at our site because of the preponderance of legumes. Within each seral stage, measurements were made on three representative trees for each species giving us a total of 18 trees per seral stage. The diameter at breast height (DBH) was measured for all trees. During the peak of the dry season, we measured midday (1200–1400 hours) bulk leaf water potentials on leafy twigs (ΨL) using a Scholander-type pressure chamber (model 1000; Plant Moisture Stress, Corvallis, OR, USA). Measurements of ΨL were made over three consecutive days, with measurements from trees in each seral stage taken each day to reduce any bias associated with sampling dates. For stable isotope analyses of xylem water, suberized twigs approximately 10 mm in diameter and 50–80 mm long were cut from individual trees. Stem samples were collected at 150 cm height or from the lower branches in taller trees to minimize any bias based on location within the canopy. Clipped twigs were immediately placed in a capped vial, wrapped in parafilm and stored in the freezer until water extraction for stable isotope analyses.
Soil samples were collected by inserting a 4.5-cm-diameter metal soil corer until we encountered limestone bedrock (usually <10 cm in depth). Within each seral stage, five samples were randomly collected for water content determination and stable isotope analyses of soil water. To characterize all possible water sources, bedrock samples were also collected from two local wells within the Reserve. Bedrock samples were collected at least 15 cm deep (horizontally) into the sidewalls of the wells to minimize the alteration of water isotopic composition by evaporative enrichment. Samples were collected using a hand-held chisel at 25, 50, 75, 100, 150, 200 and 300 cm depths. From each well, two samples were collected at each depth from opposite sidewalls. Freshly collected soil and bedrock samples were placed in capped vials, wrapped in parafilm and stored in the freezer until water extraction for stable isotope analyses. Three groundwater samples were also collected from each well.
Stable isotope analyses
Water was extracted from plant and soil/bedrock samples using a cryogenic vacuum distillation line (Ehleringer et al. 2000). Soil water content was calculated as [(fresh weight − dry weight)/dry weight × 100] according to differences in sample weight before and after thorough water extraction (100°C, 3 h). δ18O values were obtained by equilibration of 50 μl of the extracted water with 2% CO2 for 48 h. The isotopic composition of the CO2 gas was then determined using an isotope ratio mass spectrometer running in the continuous flow mode (Finnigan MAT Delta Plus XL; Thermo Instruments, Bremen, Germany) at the Center for Stable Isotope Biogeochemistry of the University of California at Berkeley. All δ18O values are expressed in delta notation (‰) relative to the internationally accepted standard (Vienna Standard Mean Oceanic Water, VSMOW). Analytical precision of the δ18O measurements was 0.22‰.
In late April 2007, sunlit leaf samples were collected from individual trees included in the water source determination study for foliar δ13C and δ18O analyses. For drought-deciduous trees, we used newly formed foliar tissue produced during the dry season. Five mature leaves were collected from the top of the each individual tree and pooled into a single sample. Leaves were oven dried at 70°C for 48 h, finely ground, and analyzed for δ13C and δ18O at the Center for Stable Isotope Biogeochemistry of the University of California, Berkeley. Analysis of δ13C was performed with a PDZ Europa 20/20 isotope ratio mass spectrometer (Manchester, UK). Oxygen isotope analysis was conducted on a Finnigan MAT Delta PlusXL (Finnigan MAT, Bremen, Germany) following the methods of Farquhar et al. (1997). All δ13C are expressed in delta notation (‰) relative to the internationally accepted standard (PeeDee Belemnite, PDB). Analytical precision for carbon and oxygen isotope analyses were 0.17 and 0.23‰, respectively.
Variation in the isotope composition of source water was removed from the oxygen isotope composition of plant foliage by presenting the compositions as enrichments relative to source water (∆18Ol) as described by Barbour et al. (2000).
where δ18Ol is the oxygen isotope composition of organic matter. Because the δ18O signature of plant xylem water remains unaltered during uptake and transport within woody tissue, we assumed that the δ18O signature of source water (δ18Os) was equal to the δ18O of plant xylem water.
Statistical analyses were conducted using SPSS statistical software (v.16.0; SPSS, 2007). Soil water content and soil water δ18O values were subjected to a one-way ANOVA followed by post hoc test using Bonferroni corrections to determine significant (P < 0.05) differences among seral stages. Bulk leaf water potential, xylem water δ18O, and foliar δ13C and ∆18Ol data were subjected to a two-way ANOVA followed by post hoc tests using Bonferroni corrections to detect significant (P < 0.05) differences among trees representing contrasting leaf phenology (evergreen vs. drought-deciduous) and seral stages. Least-square regression was also used to examine relationships among measured variables.
During March and April, only 107 mm of rain was recorded representing 5% of the total rainfall during the 12 months prior to soil/bedrock sampling (Fig. 1). Gravimetric water content in the upper 300 cm of the soil/bedrock profile fluctuated between 13 and 18%, and no clear trend with soil depth was observed (Fig. 2a). The large standard errors associated with mean soil/bedrock water content values reflects the heterogeneous nature of the profile, which is composed of soil pockets interspersed within an intricate matrix of limestone bedrock with varying water holding capacity. The pronounced seasonality in rainfall favored the development of a vertical gradient in δ18O throughout the soil/bedrock profile (Fig. 2b). Average δ18O values of soil/bedrock samples decreased abruptly from an average 4.13 ± 0.33‰ at 5 cm depth to −1.93 ± 0.20‰ at 75 cm. Between 75 and 300 cm depth, bedrock samples became progressively depleted in 18O, and at 300 cm depth the average δ18O value of bedrock water was no longer significantly different than groundwater (F 1,8 = 2.47, P = 0.16). Groundwater samples yielded an average δ18O value of −4.19 ± 0.21‰.
Differences in measured variables among seral stages are summarized in Table 2. Tree height and DBH differed significantly among seral stages (P < 0.001 for both). Surface soil water content was significantly greater in the later-seral stage compared to both the early- and mid-seral stages (F 2,14 = 20.85, P < 0.001). Average surface soil water δ18O values were significantly higher in the early- and mid-seral stages relative to the late-seral stage (F 2,14 = 6.74, P = 0.01). Trees in the early-seral stage had significantly lower ΨL values compared to trees in the late-seral stage (F 2,48 = 3.86, P = 0.03). Trees in the mid-seral stage had significantly lower xylem water δ18O values compared to trees in the early-seral stage (F 2,48 = 4.07, P = 0.02). Foliar δ13C values were significantly higher for trees in the early-seral stage relative to trees in the mid- and late-seral stage (F 2,48 = 9.12, P < 0.001). Trees in the early-seral stage had significantly higher ∆18Ol values compared to trees in the late-seral stage (F 2,48 = 3.52, P = 0.04).
Bulk leaf water potential did not differ between evergreen and drought-deciduous trees within any of the seral stages (P > 0.05; Fig. 3a). Differences in xylem water δ18O between evergreen and drought-deciduous trees were only significant in the early-seral stage (P < 0.05), whereas no differences were detected in the mid- and late-seral stages (Fig. 3b). When comparing among drought-deciduous trees, xylem water δ18O values were significantly lower in the mid- and late-seral stages relative to the early seral stage (P < 0.05, Fig. 3b). We also found a negative correlation between DBH and xylem water δ18O values among the drought-deciduous trees (r 2 = 0.15, P = 0.05), suggesting that larger, drought-deciduous trees had access to deeper water sources. For evergreen trees, xylem water δ18O values did not differ among seral stages (P > 0.05; Fig. 3b), nor was there a significant relationship between DBH and xylem water δ18O values (r 2 = 0.02, P = 0.54).
As observed for xylem water δ18O values, differences in foliar δ13C between evergreen and drought-deciduous trees were only significant in the early-seral stage (P < 0.05; Fig. 3c). When comparing among drought-deciduous trees, foliar δ13C values were significantly lower in the mid- and late-seral stages relative to the early-seral stage (P < 0.05); whereas foliar δ13C values for evergreen trees did not differ among seral stages (P > 0.05; Fig. 3c). We found a positive correlation between stem water δ18O and foliar δ13C values among the drought-deciduous species (r 2 = 0.16, P = 0.04), suggesting that drought-deciduous trees using shallower water sources had greater WUE.
Differences in ∆18Ol between evergreen and drought-deciduous trees were only significant in the early-seral stage (P < 0.05), whereas no differences were detected in the mid- and late-seral stages (P > 0.05; Fig. 3d). When comparing among drought-deciduous trees, there was a general trend of decreasing ∆18Ol values among the seral stages, which resulted in significantly lower ∆18Ol values in the late-seral stage relative to the early-seral stage (P < 0.05; Fig. 3d). When combining all seral stages, we found a positive correlation between foliar δ13C and ∆18Ol values among drought-deciduous trees (r 2 = 0.23, P = 0.01; Fig. 4). However, when comparing within individual seral stages, the relationship between foliar δ13C and ∆18Ol values was only significant for drought-deciduous species in the mid-seral stage (r 2 = 0.46, P = 0.04).
Results from this study suggest that trees in different seral stages experience significant differences in soil water availability during the dry season. In general, it appeared that trees in the early-seral stage experienced lower soil water availability relative to trees in the late-seral stage. Although there were no differences in ΨL between evergreen and drought-deciduous trees, results from stable isotope analyses suggest that evergreen and drought-deciduous trees use different strategies to overcome seasonal water limitations, especially in the early-seral stage with lower soil water availability. Based on differences in xylem water δ18O values, it appeared that evergreen trees used deeper sources of water compared to drought-deciduous trees in the early-seral stage. In contrast, higher foliar δ13C and ∆18Ol values for drought-deciduous trees relative to evergreen trees in the early-seral stage suggested that drought-deciduous species had greater WUE as a result of reduced stomatal conductance during the dry season.
Based on the combination of lower surface soil water content, lower ΨL, and high foliar δ13C values, trees in the early-seral stage appeared to operate under greater water deficit than trees in the late-seral stage. Given the close proximity and interspersed structure of the seral stages, it is unlikely that differences in surface soil water content resulted from differential precipitation among seral stages. However, extremely high density of trees <1 cm DBH combined with greater root biomass in the early-seral stage (Vargas et al. 2008) create greater demands on surface soil moisture, consistent with our finding of lower soil water content in the early-seral stage (Table 2). High δ18O values of surface soil water in the early-seral stage indicated increased rates of evaporation from the soil surface (Allison 1982; Allison and Hughes 1983). Allison and Hughes (1983) previously showed that water loss from the soil surface was greater beneath mallee (native Eucalyptus scrub) than at a cropped site in southern Australia. The authors explained the differences in water loss as the result of a denser plant cover at the cropped site, making evaporation from the soil surface less likely. Similarly, the reduced canopy stature (Allen et al. 2003b) and greater canopy openness (Vargas et al. 2009) in the early-seral stage would have led to increased rates of soil water evaporation, as indicated by high surface soil water δ18O values (Table 2). Vargas and Allen (2008) also found increasing surface evaporation following defoliation caused by Hurricane Wilma at the same site.
At the peak of the dry season, we observed a vertical isotopic gradient (δ18O) throughout the soil/bedrock profile (Fig. 2), which in turn allowed us to infer water sources used by trees. Contrary to our initial expectation, trees in the late-seral stage did not appear to use deeper sources of water than trees in the early-seral stage. The lack of difference in water sources between trees in the early- and late-seral stages may, in part, be related to differences in the amount of water stored in the shallow soils. Surface soil water content was roughly two times greater in the late-seral stage relative to the early-seral stage (Table 2), and therefore represented a substantial source of water for trees in the late-seral stage. Additionally, because the most limiting nutrients for plant growth are greatest in the upper portion of the soil profile (Jobbágy and Jackson 2001), soil water partitioning in late-seral trees may reflect a necessary trade-off resulting from higher nutrient demand associated with maintenance of an extensive canopy.
Only in the early-seral stage did we observe vertical partitioning of water sources among evergreen and drought-deciduous trees with evergreen trees using deeper sources of water than drought-deciduous trees (Fig. 3b). Similarly, Jackson et al. (1995) and Meinzer et al. (1999) have shown evergreen species tapping deeper water sources compared to drought-deciduous species in a SDTF in Panama. It is therefore possible that deep roots may free evergreen species from the constraint of shedding their leaves during the dry season. Interestingly, there was no correlation between tree size (as measured by DBH) and xylem water δ18O values for evergreen trees, which is consistent with previous studies in SDTF (Jackson et al. 1999; Querejeta et al. 2007). Jackson et al. (1999) hypothesized that rapid development of deep roots may facilitate the establishment and survival of small trees by allowing them to maintain relatively constant rates of water use. Meinzer et al. (1999) reported that smaller trees preferentially tapped deeper sources of water relative to larger trees, and conjectured that the presence of deep roots in smaller trees would substantially reduce the probability of mortality during the dry season. Thus, our finding of significantly lower xylem water δ18O values for evergreen species in the early-seral stage combined with the lack of correlation between tree DBH and xylem water δ18O values provides further evidence for the importance of evergreen species to rapidly develop deep roots as a strategy to overcome seasonal water limitations.
Significantly higher foliar δ13C values for trees in the early-seral stage relative to trees in the mid- and late-seral stage could, in part, be explained by differences in microclimate, leading to variation in soil moisture and vapour pressure deficit (VPD) among the seral stages (Table 2). Korol et al. (1999) found a positive correlation between foliar δ13C and mean annual VPD, suggesting that trees responded to a drier environment by stomatal closure, whereas stomata remained open in environments with higher humidity. Ehleringer and Copper (1988) showed that foliar δ13C values increased with decreasing water availability along a soil moisture gradient in the Sonoran Desert, indicating that WUE increased as soil water availability decreased. At our site, Goode and Allen (2009) previously reported higher VPD in the early-seral stage relative to the late-seral stage (0.60 and 0.46 kPa, respectively). Furthermore, Goode et al. (2010) found that epiphytes in the late-seral stage exhibited bulk leaf tissue and extractable sugars that were depleted in 13C, indicating lower VPD and possibly a greater shift from crassulacean acid metabolism to increasing C3 photosynthesis relative to epiphytes in early-seral stages at this site. Thus, the combination of higher VPD and lower soil moisture in the early-seral stage might be the cause for higher foliar δ13C values in this seral stage.
Drought-deciduous trees in the early-seral stage had the greatest WUE, as indicated by the highest foliar δ13C values, and the lowest stomatal conductance, as indicated by the highest ∆18Ol values, among all seral stages (Fig. 3c, d). In contrast, drought-deciduous trees in the late-seral stage had the lowest WUE, as indicated by the lowest foliar δ13C, and the highest stomatal conductance, as indicated by the lowest ∆18Ol values (Fig. 3c, d). Drought-deciduous trees in the mid-seral stage had stomatal conductance and WUE values that fell inbetween those found in the early- and late-seral stages. Furthermore, when combining drought-deciduous trees for all seral stages, we found a positive correlation between foliar δ13C and ∆18Ol (Fig. 4), suggesting that greater WUE was the result of reduced stomatal conductance. According to the conceptual model developed by Scheidegger et al. (2000), high foliar δ13C values for drought-deciduous trees, in combination with higher tissue δ18O values, can be interpreted as evidence for enhanced water stress and stomatal limitation to photosynthesis (Sternberg et al. 1989). It is important to note that several factors other than leaf level gas exchange, especially variation in mesophyll conductance and mean VPD, could contribute to such isotopic variability (Seibt et al. 2008). However, in this study, it appeared that for drought-deciduous trees stomatal regulation is an important strategy for minimizing evaporative water loss during the dry season.
In conclusion, we have shown that trees on the northern Yucatan Peninsula experience a wide range of soil water availability during secondary succession. Moreover, it appeared that evergreen and drought-deciduous trees use different strategies to overcome seasonal water limitations, which in turn may decrease competition and promote tree species coexistence in the dry tropical climate on the northern Yucatan Peninsula. These differences were especially pronounced in the early-seral stage with lower soil water availability, whereas we found no differences between evergreen and drought-deciduous trees in the mid- and late-seral stages. In the early seral stage, evergreen trees appeared to use deeper sources of water as a strategy to overcome seasonal water limitations. Drought-deciduous trees, on the other hand, in addition to losing their leaves, had reduced stomatal conductance that resulted in greater WUE of remaining leaves as a strategy to overcome water limitations during the dry season.
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We thank El Eden Ecological Reserve for their continued support and access to their research facilities. We are also grateful to Laurel Goode and Juan Castillo for their help in the field. This research was supported by grants from National Science Foundation awarded to MF Allen (DEB-9981607, DEB-0615427, and EF-0410408) and LS Santiago (DEB-0770 6813), and from a University of California Institute for Mexico and the United States dissertation research grant awarded to N. J. Hasselquist.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Communicated by Frederick C. Meinzer.
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Hasselquist, N.J., Allen, M.F. & Santiago, L.S. Water relations of evergreen and drought-deciduous trees along a seasonally dry tropical forest chronosequence. Oecologia 164, 881–890 (2010). https://doi.org/10.1007/s00442-010-1725-y
- Resource partitioning
- Stable isotopes
- Water-use efficiency
- Yucatan Peninsula