Abstract
The tropical high Andes experience greater daily temperature oscillations compared to seasonal ones as well as a high frequency of night frost occurrence year round. Survival of organisms, under such environmental conditions, has been determined by selective forces which have evolved into adaptations including avoidance or tolerance to freezing. These adaptations have been studied in different species of trees, shrubs and perennial herbs in páramo ecosystems, while they have not been considered in grasses, an important family of the páramo. In order to understand survival of Poaceae, resistance mechanisms were determined. The study was performed along an altitudinal gradient (2,500–4,200 m a.s.l.) in the páramo. Supercooling capacity and frost injury temperature were determined in nine species in order to establish cold resistance mechanisms. Grasses registered a very low supercooling capacity along the altitudinal gradient, with ice formation between −6 and −3°C. On the other hand, frost injury temperature oscillated between −18 and −7°C. Our results suggest that grasses exhibit freezing tolerance as their main cold resistance mechanism. Since grasses grow at ground level, where greatest heat loss takes place, tolerance may be related to this life form as reported for other small life forms.
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References
Alvizu PE (2004) Complejidad y respuesta funcional de la vegetación de páramo a lo largo de gradientes altitudinales. PhD dissertation. Postgrado en Ecología Tropical, ICAE, Universidad de Los Andes, Mérida
Andrews CJ (1996) How do plants survive ice? Ann Biol 78:529–536
Azócar A, Monasterio M (1980) Caracterización ecológica del clima en el páramo de Mucubají. In: Monasterio M (ed) Estudios ecológicos en los páramos andinos. Universidad de Los Andes, Mérida, pp 207–223
Azócar A, Rada F, Goldstein G (1988) Freezing tolerance in Draba chionophila, a “miniature” caulescent rosette species. Oecologia 75:156–160
Beck E (1994) Cold tolerance in tropical alpine plants. In: Rundel P, Smith AP, Meinzer F (eds) Tropical alpine environments: plant form and function. Cambridge University Press, UK pp 77–110
Beck E, Schulze E, Senser M, Scheibe R (1984) Equilibrium freezing of leaf water and extracellular ice formation in Afroalpine “giant rosette” plants. Planta 162:276–282
Beck E, Senser M, Scheibe R, Steiger H, Pongratz P (1982) Frost avoidance and freezing tolerance in Afroalpine “giant rosette” plants. Plant Cell Environ 5:215–222
Earnshaw M, Carver K, Gunn T, Kerenga K, Harvey V, Griffiths H, Broameadow M (1990) Photosynthetic pathway, chilling tolerance and cell sap osmotic potencial values of grasses along an altitudinal gradient in Papua New Guinea. Oecologia 84:280–288
Goldstein G, Rada F, Azócar A (1985) Cold hardiness and supercooling along an altitudinal gradient in Andean giant rosette species. Oecologia 68:147–152
Hedberg O (1964) Features of afroalpine plant ecology. Acta Phytogeogr Suec 49:1–144
Körner C (1999) Alpine plant life: functional plant ecology of high mountain ecosystems. Springer, Berlin Heidelberg New York
Kubien DS, Caemmerer S, Furbank RT, Sage RF (2003) C4 photosynthesis at low temperature. A study using transgenic plants with reduced amounts of Rubisco. Plant Physiol 132:1577–1585
Larcher W (1995) Physiological plant ecology, 3rd edn. Springer, Berlin Heidelberg New York
Levitt J (1972) Responses of plants to environmental stresses. Academic Press, London
Long S (1983) C4 photosynthesis at low temperatures. Plant Cell Environ 6:345–363
Márquez E (2002) Distribución altitudinal de gramíneas de páramo como respuesta a las rutas metabólicas y los mecanismos de resistencia a las bajas temperaturas. MSc dissertation. Postgrado en Ecología Tropical, ICAE, Universidad de Los Andes, Mérida
Márquez E, Fariñas MR, Briceño B, Rada F (2004) Distribution of grasses along an altitudinal gradient in a Venezuelan paramo. Rev Chil Hist Nat 77:649–660
Melcher P, Goldstein G, Meinzer F, Minyard B, Giambelluca T, Lope L (1994) Determinants of thermal balance in the Hawaiian giant rosette plant, Argyroxiphium sandwicense. Oecologia 98:412–418
Pearcy RS (2001) Plant freezing damage. Ann Bot 87:417–424
Pittermann J, Sage RF (2001) The response of the high altitude C4 grass Muhlenbergia montana (Nutt.) A.S. Hitchc. to long- and short-term chilling. J Exp Bot 52:829–838
Rada F, Goldstein G, Azócar A, Meinzer F (1985a) Freezing avoidance in Andean giant rosette plants. Plant Cell Environ 8:501–507
Rada F, Goldstein G, Azócar A, Meinzer F (1985b) Daily and seasonal osmotic changes in a tropical treeline species. J Exp Bot 36:989–1000
Rada F, Goldstein G, Azócar A, Torres F (1987) Supercooling along an altitudinal gradient in Espeletia schultzii, a caulescent giant rosette species. J Exp Bot 38:491–497
Rundel P (1994) Tropical alpine climates. In: Rundel P, Smith AP, Meinzer F (eds) Tropical alpine environments: plant form and function. Cambridge University Press, UK, pp 21–44
Sage R (2002) Variation in the k cat of Rubisco in C3 and C4 plants and some implications for photosynthetic performance at high and low temperature. J Exp Bot 53:609–620
Sage R, Sage T (2002) Microsite characteristics of Muhlenbergia richardsonis (Trin.) Rydb., an alpine C4 grass from the White Mountains, California. Oecologia 132:501–508
Sage RF, Kubien DS (2003) Quo vadis C4 ? An ecophysiological perspective on global change and the future of C4 plants. Photosyn Res 77:209–225
Sakai A, Larcher W (1987) Frost survival of plants: responses and adaptation to freezing stress. Springer Berlin Heidelberg New York
Sarmiento G (1986) Ecological features of climate in high tropical mountains. In: Vuillemier F, Monasterio M (eds) High altitude tropical biogeography. Oxford University Press, Oxford, pp 11–45
Schwarz A, Redmann R (1987) C4 grasses from the boreal forest region of northwestern Canada. Can J Bot 66:2424–2430
Squeo F, Rada F, Azócar A, Goldstein G (1991) Freezing tolerance and avoidance in high tropical Andean plants: is it equally represented in species with different plant height? Oecologia 86:378–382
Squeo F, Rada F, García C, Ponce M, Rojas A, Azócar A (1996) Cold resistance mechanisms in high desert Andean plants. Oecologia 105:552–555
Steponkus P, Lanphear F (1967) Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol 42:1423–1426
Acknowledgements
This project was supported by IAI (N° CRN-040), FONACIT (N° 98003404) and CDCHT (C-1090-01-01-E) grants. We are grateful to P. Alvizu for detailed temperature information for the gradient, A. Nieto and C. Erazo for their field assistance and A. Azócar, B. Lewis and anonymous reviewers for their comments and suggestions regarding the manuscript.
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Communicated by Jim Ehleringer.
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Márquez, E.J., Rada, F. & Fariñas, M.R. Freezing tolerance in grasses along an altitudinal gradient in the Venezuelan Andes. Oecologia 150, 393–397 (2006). https://doi.org/10.1007/s00442-006-0556-3
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DOI: https://doi.org/10.1007/s00442-006-0556-3