Oecologia

, Volume 143, Issue 4, pp 537–547 | Cite as

Genotype × environment interactions, stoichiometric food quality effects, and clonal coexistence in Daphnia pulex

  • Lawrence J. Weider
  • Wataru Makino
  • Kumud Acharya
  • Karen L. Glenn
  • Marcia Kyle
  • Jotaro Urabe
  • James J. Elser
Population Ecology

Abstract

The role of stoichiometric food quality in influencing genotype coexistence and competitive interactions between clones of the freshwater microcrustacean, Daphnia pulex, was examined in controlled laboratory microcosm experiments. Two genetically distinct clones of D. pulex, which show variation in their ribosomal (r)DNA structure, as well as differences in a number of previously characterized growth-rate-related features (i.e., life-history features), were allowed to compete in two different arenas: (1) batch cultures differing in algal food quality (i.e., high vs. low carbon:phosphorus (C:P ratio) in the green alga, Scenedesmus acutus); (2) continuous flow microcosms receiving different light levels (i.e., photosynthetically active radiation) that affected algal C:P ratios. In experiment 1, a clear genotype × environment interaction was determined with clone 1 out-competing clone 2 under high nutrient (i.e., low food C:P) conditions, while the exact opposite pattern was observed under low nutrient (i.e., high C:P) conditions. In experiment 2, clone 1 dominated over clone 2 under high light (higher C:P) conditions, but clonal coexistence was observed under low light (low C:P) conditions. These results indicate that food (nutrient) quality effects (hitherto an often overlooked factor) may play a role in microevolutionary (genotypic) responses to changing stoichiometric conditions in natural populations.

Keywords

Nutrients Elemental ratios Daphniids Competition Clones 

Notes

Acknowledgements

This work was supported by the National Science Foundation (US, Grant no. 9977047) and a Grant-in-Aid for Scientific Research B (no. 12440218) from MEXT, Japan. We thank S. Ishida, T. Ishikawa, T. Iwata, N. Kuwae, J. Togari, and C. Yoshimizu for laboratory assistance. All experiments were in compliance with applicable laws of the US and Japan. We thank S. Kohler and two anonymous reviewers for their comments.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lawrence J. Weider
    • 1
  • Wataru Makino
    • 3
    • 4
  • Kumud Acharya
    • 2
    • 5
  • Karen L. Glenn
    • 1
  • Marcia Kyle
    • 2
  • Jotaro Urabe
    • 3
    • 4
  • James J. Elser
    • 2
  1. 1.Department of Zoology and Biological StationUniversity of OklahomaNormanUSA
  2. 2.School of Life SciencesArizona State UniversityTempeUSA
  3. 3.Center for Ecological ResearchKyoto UniversityOtsuJapan
  4. 4.Graduate School of Life Science, Department of Ecology and Evolutionary BiologyTohoku UniversityAoba, SendaiJapan
  5. 5.Department of BiologyUniversity of LouisvilleLouisvilleUSA

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