, Volume 134, Issue 3, pp 317–324

Root responses and nitrogen acquisition by Artemisia tridentata and Agropyron desertorum following small summer rainfall events

  • Carolyn Y. Ivans
  • A. Joshua Leffler
  • Usha Spaulding
  • John M. Stark
  • Ronald J. Ryel
  • Martyn M. Caldwell


Resources in the Great Basin of western North America often occur in pulses, and plant species must rapidly respond to temporary increases in water and nutrients during the growing season. A field study was conducted to evaluate belowground responses of Artemisia tridentata and Agropyron desertorum, common Great Basin shrub and grass species, respectively, to simulated 5-mm (typical summer rain) and 15-mm (large summer rain) summer rainfall events. The simulated rainfall was labeled with K15NO3 so that timing of plant nitrogen uptake could be monitored. In addition, soil NH4+ and NO3 concentrations and physiological uptake capacities for NO3 and NH4+were determined before and after the rainfall events. Root growth in the top 15 cm of soil was monitored using a minirhizotron system. Surprisingly, there was no difference in the amount of labeled N acquired in response to the two rainfall amounts by either species during the 7-day sample period. However, there were differences between species in the timing of labeled N uptake. The N label was detected in aboveground tissue of Agropyron within 1 h of the simulated rainfall events, but not until 24 h after the rainfall in Artemisia. For both Agropyron and Artemisia, root uptake capacity was similarly affected by the 5-mm and 15-mm rainfall. There was, however, a greater increase in uptake capacity for NH4+ than for NO3, and the 15-mm event resulted in a longer response. No root growth occurred in either species in response to either rainfall event during this 8-day period. The results of this study indicate that these species are capable of utilizing nitrogen pulses following even small summer rainfall events during the most stressful period of the summer and further emphasize the importance of small precipitation events in arid systems.


Agropyron desertorum Artemisia tridentata Great Basin Nitrogen uptake capacity Root responses 


  1. Allen MF, Richards JH, Busso CA (1989) Influence of clipping and soils status on vesicular-arbuscular mycorrhizae of two semi-arid tussock grasses. Biol Fertil Soils 8:289–295Google Scholar
  2. BassiriRad H, Caldwell MM (1992a) Temporal changes in root growth and N-15 uptake and water relations of 2 tussock grass species recovering from water stress. Physiol Plant 86:525–531CrossRefGoogle Scholar
  3. BassiriRad H, Caldwell MM (1992b) Root growth, osmotic adjustment and NO3¯ uptake during and after a period of drought in Artemisia tridentata. Aust J Plant Physiol 19:493–500 Google Scholar
  4. BassiriRad H, Tremmel DC, Virginia A, Reynolds JF, de Soyza AG (1999) Short-term patterns in water and nitrogen acquisition by two desert shrubs following a simulated summer rain. Plant Ecol 145:27–36CrossRefGoogle Scholar
  5. Birch HF (1960) Nitrification in soils after different periods of dryness. Plant Soil 12:81–96Google Scholar
  6. Bloom AJ, Caldwell RM (1988) Root excision decreases nutrient absorption and assimilation by barley. Plant Physiol 99:1294–1301Google Scholar
  7. Brady DJ, Wenzel CL, Fillery IRP, Gregory PJ (1995) Root growth and nitrate uptake by wheat (Triticum aestivum L.) following wetting of dry surface soil. J Exp Bot 46:557–564Google Scholar
  8. Caldwell MM, Dawson TE, Richards JH (1998) Hydraulic lift: consequences of water efflux from the roots of plants. Oecologia 113:151–161CrossRefGoogle Scholar
  9. Cody RP, Smith JK (1991) Applied statistics and the SAS programming language, 3rd edn. Prentice-Hall, Englewood Cliffs, N.J. Google Scholar
  10. Cui M, Caldwell MM (1997) A large ephemeral release of nitrogen upon wetting a dry soil and corresponding root responses in the field. Plant Soil 191:291–299CrossRefGoogle Scholar
  11. Davidson EA, Matson PA, Vitousek PM, Riley R, Dunkin K, Garcia-Mendez G, Maass JM (1993) Processes regulating soil emissions of NO and N2O in a seasonally dry tropical forest. Ecology 74:130–139Google Scholar
  12. Fisher FM, Zak JC, Cunningham GL, Whitford WG (1987) Water and nitrogen effects on growth and allocation patterns of creosote bush in the northern Chihuahuan Desert. J Range Manage 41:387–391Google Scholar
  13. Gebauer RLE, Ehleringer JR (2000) Water and nitrogen uptake patterns following moisture pulses in a cold desert community. Ecology 81:1415–1424Google Scholar
  14. Goldberg D, Novoplansky A (1997) On the relative importance of competition in unproductive environments. J Ecol 85:409–418Google Scholar
  15. Gutierrez JR, Whitford WG (1987) Chihuahuan desert annuals: importance of water and nitrogen. Ecology 68:2032–2045Google Scholar
  16. Hunt ER Jr, Zakir NJD, Nobel PS (1987) Water cost and water revenues for established and rain-induced roots of Agave deserti. Funct Ecol 1:125–129Google Scholar
  17. Jackson LE, Schimel JP, Firestone MK (1989) Short-term partitioning of ammonium and nitrate between plants and microbes in an annual grassland. Soil Biol Biochem 21:409–415CrossRefGoogle Scholar
  18. Jackson RB, Manwaring JH, Caldwell MM (1990) Rapid physiological adjustment of roots to localized soil enrichment. Nature 344:58–60Google Scholar
  19. Jasper DA, Abbott LK, Robson AD (1993) The survival of infective hyphae of vesicular-arbuscular mycorrhizal fungi in dry soil: an interaction with sporulation. New Phytol 124:473–479Google Scholar
  20. Johanson A, Jakobsen I, Jensen ES (1993) External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L, 3. Hyphal transport of 32P and 15N. New Phytol 124:61–68Google Scholar
  21. Laurenroth WK, Sala OE, Milchunas DG, Lathrop RW (1987) Root dynamics of Bouteloua gracilis during short-term recovery from drought. Funct Ecol 1:117–124Google Scholar
  22. Mulvaney RL (1996) Nitrogen-inorganic forms. In: Sparks RL (ed) Methods of soil analysis, part 3. Chemical methods. ASA-SSSA, Madison, Wis., pp 1123–1200Google Scholar
  23. Nobel PS, Sanderson J (1984) Rectifier-like activities of roots of two desert succulents. J Exp Bot 35:727–737Google Scholar
  24. North GB, Nobel PS (1997) Root-soil contact for the desert succulent Agave deserti in wet and drying soil. New Phytol 135:21–29CrossRefGoogle Scholar
  25. Noy-Meir I (1973) Desert ecosystems, environment and producers. Annu Rev Ecol Syst 4:25–41Google Scholar
  26. Nye PH, Tinker PB (1977) Solute movement in the soil-root system. University of California Press, Berkeley, Calif.Google Scholar
  27. Paul EA, Clark FE (1989) Soil microbiology and biochemistry. Academic, San Diego, Calif.Google Scholar
  28. Reid CPP (1974) Assimilation, distribution, and root exudation of 14C by ponderosa pine seedlings under induced water stress. Plant Physiol 54:44–49Google Scholar
  29. Rundel PW, Jarrell WM (1989) Water in the environment. In: Pearcy RW, Ehleringer J, Mooney HA, Rundel PW (eds) Plant physiological ecology. Chapman and Hall, London, pp 29–56Google Scholar
  30. Ryel RJ, Caldwell MM (1998) Nutrient acquisition from soils with patchy nutrient distributions as assessed with simulation models. Ecology 79:2735–2744Google Scholar
  31. Ryel RJ, Caldwell MM, Yoder CK, Or D, Leffler AJ (2002) Hydraulic redistribution in a stand of Artemisia tridentata: evaluation of benefits to transpiration assessed with a simulation model. Oecologia 130:173–184Google Scholar
  32. Sala OE, Lauenroth WK (1982) Small rainfall events: an ecological role in semiarid regions. Oecologia 53:301–304Google Scholar
  33. Sala OE, Lauenroth WK, Parton WJ (1982) Plant recovery following prolonged drought in a shortgrass steppe. Agric Meteorol 27:49–58Google Scholar
  34. Sokal RR, Rohlf FJ (1994) Biometry, 3rd edn. Freeman, San FranciscoGoogle Scholar
  35. Van Genuchten MT (1980) A closed-form equation for predicting the hydraulic conductivity of unsaturated soils. J Soil Sci Soc Am 44:892–898Google Scholar
  36. Vitousek PM, Gosz JR, Grier CC, Melillo JM, Reiners WA (1982) A comparative analysis of potential nitrification and nitrate mobility in forest ecosystems. Ecol Monogr 52:155–177Google Scholar
  37. West NE (1991) Nutrient cycling in soils of semi arid and arid regions. In: Skujins J (ed) Semi-arid lands and deserts: soil resource and reclamation. Dekker, New York, pp 295–332Google Scholar
  38. Wilson SD, Tilman D (1991) Components of plant competition along an experimental gradient of nitrogen. Ecology 72:1050–1065Google Scholar
  39. Witkamp M (1969) Environmental effects on microbial turnover of some mineral elements. Soil Biol Biochem 1:167–184Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Carolyn Y. Ivans
    • 1
    • 2
    • 4
  • A. Joshua Leffler
    • 1
    • 2
  • Usha Spaulding
    • 3
  • John M. Stark
    • 3
  • Ronald J. Ryel
    • 1
    • 2
  • Martyn M. Caldwell
    • 1
    • 2
  1. 1.Department of Forest, Range, and Wildlife SciencesUtah State UniversityLoganUSA
  2. 2.The Ecology CenterUtah State UniversityLoganUSA
  3. 3.Department of BiologyUtah State UniversityLoganUSA
  4. 4.Department of Biological SciencesEastern Kentucky UniversityRichmondUSA

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