Variation in nitrogen-15 natural abundance and nitrogen uptake traits among co-occurring alpine species: do species partition by nitrogen form?

Abstract

In the N-limited alpine tundra, plants may utilize a diversity of N sources (organic and inorganic N) in order to meet their nutritional requirements. To characterize species-level differences in traits related to N acquisition, we analyzed foliar δ¹⁵N, nitrate reductase activity (NRA) and mycorrhizal infection in co-occurring alpine species during the first half of the growing season and compared these traits to patterns of N uptake using a ¹⁵N (¹⁵N-NH₄ ⁺, ¹⁵N-NO₃ ^–) or ¹³C,¹⁵N ([1]-¹³C-¹⁵N-glycine) tracer addition in the greenhouse. ¹³C enrichment in belowground tissue indicated that all species were capable of taking up labeled glycine, although only one species showed uptake of glycine potentially exceeding that of inorganic N. Species showing the most depleted foliar δ¹⁵N and elevated NRA in the field also tended to show relatively high rates of NO₃ ^– uptake in the greenhouse. Likewise, species showing the most enriched foliar δ¹⁵N also showed high rates of NH₄ ⁺ uptake. The ratio of NO₃ ^–:NH₄ ⁺ uptake rates and growth rate explained 64% and 72% of the variance in foliar δ¹⁵N, respectively, suggesting that species differ in the ability to take up NO₃ ^– and NH₄ ⁺ in the field and that such differences may enable species to partition soil N on the basis of N form.