GABA uptake and phenotypic characteristics of the subcommissural ependymocytes of the semi-desertic rodent, Meriones shawi: correlation with serotoninergic innervation

Abstract.

Many studies have emphasized species differences in the serotoninergic innervation and phenotypic characteristics of the subcommissural organ in mammals. The post-natal distribution patterns of serotonin-containing fibers, the onset of gamma-aminobutyric acid uptake, and glial markers have been studied in the subcommissural organ of the semi-desertic rodent, Meriones shawi, by using immunohistochemical and autoradiographic techniques. Abundant serotoninergic fibers can be observed in the subcommissural organ of the newborn Meriones, some of them running among the ependymocytes and reaching the apical part of this organ. During the first 2 post-natal weeks of development, the subcommissural organ displays a progressive increase of serotonin fiber density throughout the organ, including the apical part. The existence of a dense serotonin-containing basal plexus concomitantly with a high apical innervation in this organ is a specific characteristic of Meriones. Ependymocytes of this organ have the ability to take up gamma-aminobutyric acid at birth. This uptake decreases and completely disappears from the 2nd week. The reappearance of gamma-aminobutyric acid accumulation in ependymocytes of the adult subcommissural organ after destruction of the serotonin innervation by a neurotoxin (5–7 dihydroxytryptamine) suggests an inhibitory effect of the serotonin innervation on this accumulation. Immunohistochemical studies of the phenotype of the ependymocytes with respect to glial markers during ontogeny show the transitory expression of glial fibrillary acidic protein, the presence of vimentin and the absence of S100 protein expression. No correlation has been found between the serotonin innervation and the expression of the glial markers.