Abstract
The olfactory organ of turtles consists of an upper chamber epithelium (UCE) with associated glands, and a lower chamber epithelium (LCE) devoid of glands. The UCE and LCE are referred to as the air-nose and the water-nose, respectively, because the UCE is thought to detect airborne odorants, while the LCE detects waterborne odorants. However, it is not clear how the two are used in the olfactory organ. Odorant receptors (ORs) are the major olfactory receptors in turtles; they are classified as class I and II ORs, distinguished by their primary structure. Class I ORs are suggested to be receptive to water-soluble ligands and class II ORs to volatile ligands. This study analyzed the expression of class I and II ORs in hatchlings of the green sea turtle, Chelonia mydas, through in situ hybridization, to determine the localization of OR-expressing cells in the olfactory organ. Class I OR-expressing cells were distributed mainly in the LCE, implying that the LCE is receptive to waterborne odorants. Class II OR-expressing cells were distributed in both the UCE and LCE, implying that the entire olfactory organ is receptive to airborne odorants. The widespread expression of class II ORs may increase opportunities for sea turtles to sense airborne odorants.
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Acknowledgments
We thank Haruka Sasaki for technical assistance with primer designing, tissue sectioning, and tissue processing. We thank Asahi Nakamuta for expert assistance with phylogenetic analysis.
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Conceptualization, SN, NN; Sample preparation, MM, MI, MK.; Investigation, SN; Data analysis, NN; Wrote paper, NN; Supervision, MM, YY. All authors have read and agreed to the final version of the manuscript.
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Nakamuta, S., Mori, M., Ito, M. et al. In situ hybridization analysis of olfactory receptor expression in the sea turtle olfactory organ. Cell Tissue Res 393, 253–264 (2023). https://doi.org/10.1007/s00441-023-03782-6
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DOI: https://doi.org/10.1007/s00441-023-03782-6