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Lung inflammation from repeated exposure to LPS and glyphosate

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Abstract

Agricultural workplaces consist of multiple airborne contaminants and inhalation exposures induce respiratory effects in workers. Endotoxin (LPS) and glyphosate are two common airborne contaminants in agricultural environments. We have previously shown that exposure to a combination of LPS and glyphosate synergistically modulates immune reactions as compared to individual exposures. The immunopathogenesis of acute and chronic exposure to complex agricultural exposures including LPS and glyphosate is not known; therefore, we further investigated the lung cellular inflammatory differences in mice exposed to either a combination, or individual, LPS, and glyphosate for 1 day, 5 days, and 10 days. Exposure to a combination of LPS and glyphosate resulted in greater cellular inflammatory effects in lungs as compared to individual exposures to LPS or glyphosate. Repeated exposures to the combination of LPS and glyphosate resulted in robust infiltration of inflammatory cells in the perivascular, peribronchiolar, and alveolar regions, and increases of alveolar septal thicknesses and perivascular spaces in the lungs with intense intercellular adhesion molecule (ICAM) − 1 staining in the perivascular region, but minimal staining in the pulmonary artery endothelium.

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Acknowledgements

The authors thank Dr. Aditya Manek, Heather Neufeld, and the Vivarium staff from the University of Saskatchewan for their assistance during the project. The authors pay tribute to the laboratory animals for their tremendous contribution in this project.

Funding

This study was funded by a Discovery Grant from Natural Sciences and Engineering Research Council (NSERC). Mr. Upkardeep Pandher received support from Integrated Graduate Training Program in Infectious Diseases, Food Safety and Public Policy (ITraP) funded by NSERC/CREATE, and from the University of Saskatchewan’s University Graduate Scholarship (UGS), Respiratory Research Centre Graduate Student Award (RRCGSA) from Respiratory Research Centre, Founding Chairs Fellowship from CCHSA, and Health Sciences Graduate Scholarship (HSGS).

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Authors and Affiliations

  1. Department of Medicine, College of Medicine, Canadian Centre for Health and Safety in Agriculture, University of Saskatchewan, 104 Clinic Place, P.O. Box 23, Saskatoon, SK, S7N 2Z4, Canada

    Upkardeep Pandher, Shelley Kirychuk, David Schneberger & Brooke Thompson

  2. Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada

    Gurpreet Aulakh

  3. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141004, India

    R. S. Sethi

  4. Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, SK, S7N 5B4, Saskatoon, Canada

    Baljit Singh

Authors
  1. Upkardeep Pandher
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  2. Shelley Kirychuk
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  3. David Schneberger
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  4. Brooke Thompson
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  5. Gurpreet Aulakh
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  6. R. S. Sethi
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  7. Baljit Singh
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Corresponding author

Correspondence to Shelley Kirychuk.

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The study design was approved by the Animal Research Ethics Board of the University of Saskatchewan (AUP 20160106), and animal research was conducted according to Canadian Council on Animal Care guidelines for the care and use of animals.

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Pandher, U., Kirychuk, S., Schneberger, D. et al. Lung inflammation from repeated exposure to LPS and glyphosate. Cell Tissue Res 386, 637–648 (2021). https://doi.org/10.1007/s00441-021-03531-7

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  • DOI: https://doi.org/10.1007/s00441-021-03531-7

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