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Gene expression of bovine endometrial epithelial cells cultured in matrigel

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Abstract

Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE, SERPINA14 and GRP, was substantially high in matrigel-cultured BEE than in monolayer (P  <  0.05). P4 and INFα have no significant effect on the SERPINA14 expression of BEE cultured in matrigel without co-culture with BES. On the other hand, when BEE were co-cultured with BES in matrigel culture, the expression of FGF13 was increased by the P4 treatment (P  <  0.05). Furthermore, SERPINA14 and TXN expressions were increased by P4 + IFNα treatment (P  <  0.05). These results demonstrate the appropriate conditions for BEE to form glandular structures in matrigel and the effect of co-culture with BES. The present study highlighted the possible use of matrigel for the culture of BEE to investigate the expression of cell-specific glandular epithelial genes as well as P4 and type-I IFN as factors controlling endometrial function during the implantation period.

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Funding

This work was supported by the Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (grant no. 19K22311).

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Authors and Affiliations

  1. Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan

    Daichi Nishino, Ai Kotake, Chi Sun Yun, Al-Nur Md. Iftekhar Rahman & Nobuhiko Yamauchi

  2. Department of Animal Nutrition, Genetics and Breeding, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh

    Al-Nur Md. Iftekhar Rahman

  3. Animal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Mohamed El-Sharawy

  4. Faculty of Agriculture, Saga University, Saga, 840-8502, Japan

    Ken-ichi Yamanaka

  5. Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    M. A. M. Yahia Khandoker

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  1. Daichi Nishino
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  2. Ai Kotake
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  3. Chi Sun Yun
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  4. Al-Nur Md. Iftekhar Rahman
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  5. Mohamed El-Sharawy
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  7. M. A. M. Yahia Khandoker
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  8. Nobuhiko Yamauchi
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Correspondence to Nobuhiko Yamauchi.

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The animals used in this study were treated according to the guidelines for Animal Experiments in the Faculty of Agriculture of Kyushu University (no. A19-297-0) and the laws of the Japanese Government (Law no. 105 with notification no. 6).

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Summary sentence: Gene expressions of bovine endometrial epithelial cells cultured in matrigel were analyzed. The cells showed the gland-like structure when cultured in 15% matrigel. The expression of FGF13, SEPARINA14, and TXN was increased when the cells were co-cultured in matrigel with endometrial stromal.

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Nishino, D., Kotake, A., Yun, C.S. et al. Gene expression of bovine endometrial epithelial cells cultured in matrigel. Cell Tissue Res 385, 265–275 (2021). https://doi.org/10.1007/s00441-021-03418-7

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