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The role of IL-10 in immune responses against Pseudomonas aeruginosa during acute lung infection

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Abstract

Pseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bacterium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung inflammatory response. However, this response must be controlled via immunoregulatory pathways. In this study, we evaluate the role of endogenous murine IL-10 after acute infection with the virulent strain P. aeruginosa PA14. To assess the role of IL-10, intratracheal infection with the PA14 strain was performed in C57BL/6 or IL-10 KO mice. The PA14 strain was recovered in both types of animals, although IL-10 KO mice presented a higher number of viable bacteria in the lung when compared to the C57BL/6 group. Histopathological and stereological analyses showed that IL-10 KO mice had higher tissue damage and inflammatory infiltrate when compared to control animals. The activity of MMP-9 but not MMP-2, as well as IL-6 and TNF-α expression, were augmented in the lungs of infected animals and was much more evident in IL-10 KO animals when compared to the other analyzed groups. This work indicates that endogenous IL-10 control P. aeruginosa infection, the expression of pro-inflammatory genes, MMP-9 activity and histopathological processes of the infectious process in question.

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Funding

This study was supported in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) (Finance Code 001).

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Authors and Affiliations

  1. Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil

    Valéria Aparecida Belo, Jéssica Assis Pereira, Sara Franchin D. Souza, Fernanda de Lima Tana & Leonardo Augusto de Almeida

  2. Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Brazil

    Bruna P. Pereira & Carla S. Ceron

  3. Laboratório de Biologia Molecular, Universidade Federal de São João Del-Rei (CCO), Divinópolis, Brazil

    Débora de Oliveira Lopes

  4. Departamento de Biologia Estrutural, Universidade Federal de Alfenas, Alfenas, Brazil

    Rômulo D. Novaes

  5. Laboratório de Imunologia das Doenças Infecciosas E Crônicas, Universidade José Do Rosário Vellano, Alfenas, Minas Gerais, Brazil

    Patrícia Paiva Corsetti

Authors
  1. Valéria Aparecida Belo
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  2. Jéssica Assis Pereira
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  3. Sara Franchin D. Souza
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  4. Fernanda de Lima Tana
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  5. Bruna P. Pereira
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  6. Débora de Oliveira Lopes
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  7. Carla S. Ceron
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  8. Rômulo D. Novaes
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  9. Patrícia Paiva Corsetti
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  10. Leonardo Augusto de Almeida
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Contributions

VAB, SFDS, FLT, DOL, CSC, RDN, PPC and LAA designed the project and experiments. VAB, JAP, SFDS, FLT and BPP carried out most of the experiments. VAB, JAP, RDN, PPC and LAA wrote the manuscript. VAB, SFDS, BPP and LAA carried out statistical analysis and prepared figures. LAA submitted this paper. All authors reviewed the manuscript.

Corresponding author

Correspondence to Leonardo Augusto de Almeida.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was carried out in strict accordance with the Brazilian laws on animal experimentation. The protocol was approved by the Ethics Committee on the Use of Animals at the Federal University of Alfenas, Minas Gerais, Brazil (Permit Number: CEUA no. 42/2017). The experimentation was carried out in collaboration with the Laboratory of Molecular Biology at the Federal University of São João Del-Rei, Dona Lindu Center West Campus, Minas Gerais, Brazil, which had certification CQB 301/10

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Belo, V.A., Pereira, J.A., Souza, S.F.D. et al. The role of IL-10 in immune responses against Pseudomonas aeruginosa during acute lung infection. Cell Tissue Res 383, 1123–1133 (2021). https://doi.org/10.1007/s00441-020-03308-4

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  • DOI: https://doi.org/10.1007/s00441-020-03308-4

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