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Expression of prostaglandin (PG) D synthase lipocalin and hematopoietic type and PG D receptor during restart of spermatogenesis following downregulation using a slow release GnRH agonist implant in the dog

Cell and Tissue Research volume 378, pages 359–370 (2019)Cite this article

Abstract

Prostaglandin D and the associated prostaglandin D synthases (PGDS) and receptor (DP) are considered to be involved in spermatogenesis. However, the interplay of the PGDS-DP system in male reproduction is far from being understood. The expression of PGDS lipocalin (L) and hematopoietic (H) type and DP was studied in the GnRH agonist–downregulated canine testis (week, w 0) and during recrudescence of spermatogenesis after implant removal (w 3, 6, 9, 12). H-PGDS, L-PGDS and DP were present in the adult (CG), juvenile (JG) and downregulated canine testis at the mRNA level. PGDS immunohistochemistry revealed positive staining in the cytoplasm of Leydig cells (LCs) of all samples i.e., no difference between groups. mRNA expression (ratio) of L-, H-PGDS and DP did not differ between groups w 0–12 and CG. In contrast, significant differences were found for L-PGDS (p = 0.0388), H-PGDS (p < 0.001) and DP (p < 0.001) for the groups at downregulation (w0, suprelorin group, SG, profact group, PRG) compared with the control groups (JG, CG). L-PGDS expression was lowest in JG, whereas H-PGDS was significantly lower in CG compared with JG and at downregulation (p < 0.001 to p < 0.01). The highest ratio for H-PGDS and DP was observed in the dogs treated with buserelin acetate (PRG). Our data show that the PGDS-DP system is expressed in juvenile and adult canine testes and that downregulation of the testicular endocrine and germinative function significantly affects H-PGDS, L-PGDS and DP mRNA expression indicating a role in the regulation of spermatogenesis.

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Acknowledgments

The financial support of the project by the Dr. Jutta & Dr. Georg Bruns Stiftung is gratefully acknowledged. The authors also would like to thank Dr. Gabi Itter (Sanofi-Aventis, Frankfurt) for providing the beagle dogs and Intervet Pharma R&D, Angers Technopole, France, for provision of the Gonazon® implant. Finally, we are thankful to those people who took the dogs in their custody for their great collaboration.

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Authors and Affiliations

  1. Department of Veterinary Clinical Sciences, Section for Veterinary Reproduction and Obstetrics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Hanna Körber & Sandra Goericke-Pesch

  2. Reproductive Unit of the Clinic – Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, Bünteweg 15, 30559, Hannover, Germany

    Sandra Goericke-Pesch

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  1. Hanna Körber
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  2. Sandra Goericke-Pesch
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Correspondence to Sandra Goericke-Pesch.

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All applicable national and institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted.

Animal experimentation had been approved by the respective authority (permit no. AZ V54-19c20/15c GI18/14, Regierungspräsidium Gießen).

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Körber, H., Goericke-Pesch, S. Expression of prostaglandin (PG) D synthase lipocalin and hematopoietic type and PG D receptor during restart of spermatogenesis following downregulation using a slow release GnRH agonist implant in the dog. Cell Tissue Res 378, 359–370 (2019). https://doi.org/10.1007/s00441-019-03059-x

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