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Sialoglycoprotein isolated from Carassius auratus eggs promotes osteogenesis by stimulating mesenchymal stem cells to commit to osteoblast differentiation

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Abstract

In this study, we explore whether the pro-osteogenic effects of sialoglycoprotein from Carassius auratus eggs (Ca-SGP) involve mesenchymal stem cells (MSCs). Ovariectomized osteoporotic mice treated with Ca-SGP had increased bone formation and reduced bone marrow adipose tissue. As MSCs are common progenitors of osteoblasts and adipocytes, we isolated MSCs from Ca-SGP-treated mice and found that they tended to differentiate into osteoblasts over adipocytes confirmed by Alizarin red and Oil red O staining. This change was seen at the gene and protein level. To further explore the effect of Ca-SGP on MSCs, we isolated MSCs from healthy mice and treated them with Ca-SGP in vitro. We discovered that Ca-SGP promoted MSC differentiation to osteoblasts. In addition, Ca-SGP promoted osteogenesis and reduced the fat in marrow cavity of adolescent mice. For the first time, our results demonstrate that Ca-SGP promotes osteogenesis via stimulating MSCs to commit to osteoblasts.

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Funding

This study is financially supported by the National Key R&D Program of China (No. 2018YFC0311203), National Natural Science Foundation of China (No. 31371876), Key Research & Development Plan of Shandong Province (No. 2016YYSP017) and National Key Research and Development Program (No. 2017YFF0207800).

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong Province, China

    Lei Mao, Meiling Wang, Yufeng Dai, Na Wang, Jingfeng Wang & Changhu Xue

  2. China National Institute of Standardization, Beijing, 100191, China

    Xingjun Xi

Authors
  1. Lei Mao
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  2. Meiling Wang
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  3. Xingjun Xi
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  4. Yufeng Dai
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  5. Na Wang
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  6. Jingfeng Wang
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  7. Changhu Xue
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Corresponding author

Correspondence to Jingfeng Wang.

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Animal protocols were approved by the ethical committee of experimental animal care at Ocean University of China (certificate no. SYXK20120014). All animal experiments were carried out following institutional guidelines.

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The authors declare that they have no competing interest.

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Table S1

Primers used for determination of mRNA expression. (DOC 16 kb)

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Mao, L., Wang, M., Xi, X. et al. Sialoglycoprotein isolated from Carassius auratus eggs promotes osteogenesis by stimulating mesenchymal stem cells to commit to osteoblast differentiation. Cell Tissue Res 376, 365–376 (2019). https://doi.org/10.1007/s00441-018-2976-x

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  • DOI: https://doi.org/10.1007/s00441-018-2976-x

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