In vitro remodeling and structural characterization of degradable polymer scaffold-based tissue-engineered vascular grafts using optical coherence tomography

  • Wanwen Chen
  • Junqing Yang
  • Wenjun Liao
  • Jiahui Zhou
  • Jianyi Zheng
  • Yueheng Wu
  • Dongfeng Li
  • Zhanyi Lin
Regular Article

Abstract

Non-destructive imaging strategies to monitor long-term cultures is essential for vascular engineering. The goal of this study is to investigate whether optical coherence tomography (OCT) can be a suitable approach to monitor the long-term remodeling process of biodegradable polymeric scaffold-based tissue-engineered vascular grafts (TEVG) after pulsatile stimulation and to observe polymeric scaffold degradation during bioreactor cultivation. In the present study, a perfusion system driven by a ventricular assist device was provided for a three-dimensional culture system as a pulsatile force. We characterized the structural features of wall thickness and polyglycolic acid degradation based on optical signal attenuation using catheter-based OCT. Scanning electron microscopy confirmed morphological changes. Also, polymer degradation and the detection of different types of collagen was visualized after 4 weeks of culture by means of polarized microscopy. Findings on OCT imaging correlated with those on histological examination and revealed the effects of pulsatile stimulation on the development of engineered vessels. This finding demonstrated that real-time imaging with OCT may be a promising tool for monitoring the growth and remodeling characterization of TEVG and provide a basis to promote the ideal and long-term culture of vascular tissue engineering.

Keywords

Vascular tissue engineering Polyglycolic acid Biodegradation Mechanical conditions Optical coherence tomography 

Abbreviations

bFGF

Basic fibroblast growth factor

ECM

Extracellular matrix

ePTFE

Expanded polytetrafluoroethylene

HE

Hematoxylin and eosin

NLOM

Nonlinear optical microscopy

OCT

Optical coherence tomography

PDGF

Platelet-derived growth factor

PGA

Polyglycolic acid

SEM

Scanning electron microscopy

TEVG

Tissue-engineered vascular grafts

VSMCs

Vascular smooth muscle cells

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Wanwen Chen
    • 1
  • Junqing Yang
    • 1
  • Wenjun Liao
    • 2
  • Jiahui Zhou
    • 1
  • Jianyi Zheng
    • 3
  • Yueheng Wu
    • 4
  • Dongfeng Li
    • 4
  • Zhanyi Lin
    • 1
    • 5
  1. 1.Department of Cardiology, Guangdong Academy of Medical SciencesGuangdong General HospitalGuangzhouChina
  2. 2.Department of CardiologyGuangdong NO.2 Provincial People’s HospitalGuangzhouChina
  3. 3.Department of Cardiologythe First Affiliated Hospital of Guangdong College of PharmacyGuangzhouChina
  4. 4.Medical Research Center, Guangdong Academy of Medical SciencesGuangdong General HospitalGuangzhouChina
  5. 5.Institute of Geriatric medicine, Guangdong Academy of Medical SciencesGuangdong General HospitalGuangzhouChina

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