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MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells

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Abstract

Mesenchymal stem cells (MSCs), also referred to as multipotent stromal cells, have been isolated from various adult tissue sources because of their capabilities of differentiating into multiple cell lineages including osteoblasts, thus providing a novel approach for treating bone diseases and metabolic disorders. Despite extensive potential in cell therapy and widespread interest in clinical applications of MSCs, the molecular mechanisms with regard to the regulation of their therapeutic properties and osteoblast differentiation remain to be fully elucidated. MicroRNAs (miRNAs), a novel class of endogenous small noncoding RNAs, regulate gene expressions by translational repression or degradation of their targets. Recently, emerging evidence has shown that miRNAs are closely involved in controlling the key steps of osteoblast differentiation in MSCs. This review focuses on miRNAs and their roles in regulating osteogenic differentiation of MSCs.

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Abbreviations

MSCs:

Mesenchymal stem cells

miRNAs:

MicroRNAs

RNA pol II:

RNA polymerase II

pri-miRNAs:

Primary miRNAs

pre-miRNAs:

Precursor miRNAs

RISC:

RNA-induced silencing complex

3'UTR:

3'Untranslated region

hASCs:

Human adipose tissue-derived mesenchymal stem cells

ALP:

Alkaline phosphatase

Osx:

Osterix

Dlx5:

Distal-less homeobox 5

BMP-2:

Bone morphogenetic protein-2

ChIP:

Chromatin immunoprecipitation

BMPR2:

Bone morphogenetic protein receptor type II

HDAC:

Histone deacetylase

PPARγ:

Peroxisome proliferator-activated receptor-γ

OCN:

Osteocalcin

COUP-TFII:

Chicken ovalbumin upstream promoter-transcription factor II

GSK-3:

Glycogen synthase kinase 3

hUMSCs:

Human umbilical cord mesenchymal stem cells

SFRP2:

Secreted frizzled related protein 2

Ets1:

Erythroblastosis virus E26 oncogene homolog 1

TGF-β:

Transforming growth factor-β

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Authors’ contributions

CH and SW-J participated in the design of the review. CH contributed in table designing and review writing. JN-G searched for literature and helped to design figures. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, No.1 Shizishan Street, Wuhan, Hubei, 430070, China

    Cong Huang, Junnan Geng & Siwen Jiang

  2. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China

    Cong Huang, Junnan Geng & Siwen Jiang

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  1. Cong Huang
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  2. Junnan Geng
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  3. Siwen Jiang
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Correspondence to Siwen Jiang.

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The authors declare that they have no competing interests.

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This study was supported by grants from the National Natural Science Foundation of China (nos. 31272425 and 31472075), the National Science and Technology Pillar Program (no. 2011BAD28B01), and the Major Technology Innovation Program of Hubei Province (no. 2014ABA025).

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Huang, C., Geng, J. & Jiang, S. MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells. Cell Tissue Res 368, 229–238 (2017). https://doi.org/10.1007/s00441-016-2462-2

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