Cell and Tissue Research

, Volume 368, Issue 2, pp 229–238 | Cite as

MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells

Review

Abstract

Mesenchymal stem cells (MSCs), also referred to as multipotent stromal cells, have been isolated from various adult tissue sources because of their capabilities of differentiating into multiple cell lineages including osteoblasts, thus providing a novel approach for treating bone diseases and metabolic disorders. Despite extensive potential in cell therapy and widespread interest in clinical applications of MSCs, the molecular mechanisms with regard to the regulation of their therapeutic properties and osteoblast differentiation remain to be fully elucidated. MicroRNAs (miRNAs), a novel class of endogenous small noncoding RNAs, regulate gene expressions by translational repression or degradation of their targets. Recently, emerging evidence has shown that miRNAs are closely involved in controlling the key steps of osteoblast differentiation in MSCs. This review focuses on miRNAs and their roles in regulating osteogenic differentiation of MSCs.

Keywords

Mesenchymal stem cells microRNAs Osteogenesis Transcription factors Signaling pathways 

Abbreviations

MSCs

Mesenchymal stem cells

miRNAs

MicroRNAs

RNA pol II

RNA polymerase II

pri-miRNAs

Primary miRNAs

pre-miRNAs

Precursor miRNAs

RISC

RNA-induced silencing complex

3'UTR

3'Untranslated region

hASCs

Human adipose tissue-derived mesenchymal stem cells

ALP

Alkaline phosphatase

Osx

Osterix

Dlx5

Distal-less homeobox 5

BMP-2

Bone morphogenetic protein-2

ChIP

Chromatin immunoprecipitation

BMPR2

Bone morphogenetic protein receptor type II

HDAC

Histone deacetylase

PPARγ

Peroxisome proliferator-activated receptor-γ

OCN

Osteocalcin

COUP-TFII

Chicken ovalbumin upstream promoter-transcription factor II

GSK-3

Glycogen synthase kinase 3

hUMSCs

Human umbilical cord mesenchymal stem cells

SFRP2

Secreted frizzled related protein 2

Ets1

Erythroblastosis virus E26 oncogene homolog 1

TGF-β

Transforming growth factor-β

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.The Cooperative Innovation Center for Sustainable Pig ProductionWuhanChina

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