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Spatiotemporal distribution of extracellular matrix changes during mouse duodenojejunal flexure formation

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Abstract

Although gut flexures characterize gut morphology, the mechanisms underlying flexure formation remain obscure. Previously, we analyzed the mouse duodenojejunal flexure (DJF) as a model for its formation and reported asymmetric morphologies between the inner and outer bending sides of the fetal mouse DJF, implying their contribution to DJF formation. We now present the extracellular matrix (ECM) as an important factor for gut morphogenesis. We investigate ECM distribution during mouse DJF formation by histological techniques. In the intercellular space of the gut wall, high Alcian-Blue positivity for proteoglycans shifted from the outer to the inner side of the gut wall during DJF formation. Immunopositivity for fibronectin, collagen I, or pan-tenascin was higher at the inner than at the outer side. Collagen IV and laminins localized to the epithelial basement membrane. Beneath the mesothelium at the pre-formation stage, collagen IV and laminin immunopositivity showed inverse results, corresponding to the different cellular characteristics at this site. At the post-formation stage, however, laminin positivity beneath the mesothelium was the reverse of that observed during the pre-formation stage. High immunopositivity for collagen IV and laminins at the inner gut wall mesenchyme of the post-formation DJF implied a different blood vessel distribution. We conclude that ECM distribution changes spatiotemporally during mouse DJF formation, indicating ECM association with the establishment of asymmetric morphologies during this process.

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Acknowledgments

We thank all the people involved with this work.

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Authors and Affiliations

  1. Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan

    Sawa Onouchi, Osamu Ichii, Teppei Nakamura, Yaser Hosny Ali Elewa & Yasuhiro Kon

  2. Section of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories, Chitose, Hokkaido, Japan

    Teppei Nakamura

  3. Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Yaser Hosny Ali Elewa

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  1. Sawa Onouchi
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  2. Osamu Ichii
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  3. Teppei Nakamura
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  4. Yaser Hosny Ali Elewa
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  5. Yasuhiro Kon
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Corresponding author

Correspondence to Yasuhiro Kon.

Additional information

This work was supported by the JSPS KAKENHI (grant number 2600167304). The research described in this paper was selected for the Encouragement Award at the 158th Japanese Association of Veterinary Anatomists in Aomori (September 7–9, 2015).

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Fig. S1

Comparison of CD31 positivity between the inner and outer bending sides of the mouse duodenojejunal flexure (DJF) at the post-formation stage (I inner side of the DJF, O outer side of the DJF). a Immunohistochemistry for CD31 in the mouse DJF at the post-formation stage. b Immunohistochemistry for CD34 in a serial section to that in (a) at the post-formation stage. Bars 50 μm. c Comparison of IntDen ratio for the CD31-positive reactions (asterisk significant difference between the inner and outer bending sides of the DJF as given by Wilcoxon test, P < 0.05). Values = means ± standard error; n ≥ 4 (PDF 193 kb)

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Onouchi, S., Ichii, O., Nakamura, T. et al. Spatiotemporal distribution of extracellular matrix changes during mouse duodenojejunal flexure formation. Cell Tissue Res 365, 367–379 (2016). https://doi.org/10.1007/s00441-016-2390-1

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  • DOI: https://doi.org/10.1007/s00441-016-2390-1

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