Cell and Tissue Research

, Volume 362, Issue 1, pp 87–96 | Cite as

Epigallocatechin-3-gallate-induced free-radical production upon adipogenic differentiation in bovine bone-marrow mesenchymal stem cells

  • Jin Young Jeong
  • Mi Na Park
  • Eun Seok Cho
  • Hyun-Jun Jang
  • Sungkwon Park
  • Hyun-Jeong LeeEmail author
Regular Article


Epigallocatechin-3-gallate (EGCG), a major component of catechin in green tea, has known effects on cancer, diabetes and obesity. We recently reported that the expression levels of various genes and proteins involved in adipogenesis decreases following EGCG treatment. We also assessed apoptosis in EGCG-exposed cells. Here, we explore the variability in free-radical production in bovine bone-marrow mesenchymal stem cells (BMSCs) treated with EGCG. Upon adipogenic differentiation, BMSCs were exposed to various EGCG concentrations (0, 0.1, 1, 5, or 10 μM) for 2, 4, or 6 days. We found that EGCG reduced cell viability and arrested the cell cycle at the gap 2/mitosis phase and that EGCG potentially enhanced the production of free radicals, including reactive oxygen species and reactive nitrogen species, in a concentration- and time-dependent manner. Immunostaining revealed that the expression of genes encoding CCAAT/enhancer-binding protein alpha and stearoyl-CoA desaturase were diminished by EGCG treatment. These findings suggest that EGCG alters free-radical production activity during adipogenic differentiation in BMSCs.


Adipogenic differentiation Bone-marrow mesenchymal stem cells Epigallocatechin-3-gallate Free radicals Bovine 

Supplementary material

441_2015_2191_Fig7_ESM.gif (19 kb)
Fig. S1

Cell viability was measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay during adipogenic differentiation of bovine bone-marrow mesenchymal stem cells (BMSCs). The effect of epigallocatechin-3-gallate (EGCG) on adipogenic differentiation was not concentration- or time-dependent. We observed an effect only at a high EGCG concentration (10 μM). The x-axis shows the EGCG concentrations and the y-axis shows the percentage of viable cells. Values represent means ± standard error means (SEM). The letters in superscript indicate significant between-group differences (p < 0.05). (GIF 18 kb)

441_2015_2191_MOESM1_ESM.tif (2.5 mb)
High resolution image (TIFF 2585 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Jin Young Jeong
    • 1
    • 2
  • Mi Na Park
    • 2
  • Eun Seok Cho
    • 4
  • Hyun-Jun Jang
    • 5
  • Sungkwon Park
    • 3
  • Hyun-Jeong Lee
    • 2
    Email author
  1. 1.Animal Products Utilization DivisionNational Institute of Animal Science, Rural Development AdministrationWanju-gunRepublic of Korea
  2. 2.Division of Animal Genomics and Bioinformatics, National Institute of Animal ScienceRural Development AdministrationWanju-gunRepublic of Korea
  3. 3.Department of Food Science and TechnologySejong UniversitySeoulRepublic of Korea
  4. 4.Department of Animal Resources Development Swine Science DivisionNational Institute of Animal Science, Rural Development AdminstrationCheonan-cityRepublic of Korea
  5. 5.College of PhamacyDankook UniversityCheonanRepublic of Korea

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