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The midgut of the silkmoth Bombyx mori is able to recycle molecules derived from degeneration of the larval midgut epithelium

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Abstract

The midgut represents the middle part of the alimentary canal and is responsible for nutrient digestion and absorption in insect larva. Despite the growing interest in this organ for different purposes, such as studies on morphogenesis and differentiation, stem cell biology, cell death processes and transport mechanisms, basic information on midgut development is still lacking for a large proportion of insect species. Undoubtedly, this lack of data could hinder the full exploitation of practical applications that involve midgut as their primary target. This may represent in particular a significant problem for Lepidoptera, an insect order that includes some of the most important species of high economic importance. With the aim of overcoming this fragmentation of knowledge, we performed a detailed morphofunctional analysis of the midgut of the silkworm, Bombyx mori, a representative model among Lepidoptera, during its development from the larval up to the adult stage, focusing attention on stem cells. Our data demonstrate stem cell proliferation and differentiation, not only in the larval midgut but also in the pupal and adult midgut epithelium. Moreover, we present evidence for a complex trophic relationship between the dying larval epithelium and the new adult one, which is established during metamorphosis. This study, besides representing the first morphological and functional characterization of the changes that occur in the midgut of a lepidopteron during the transition from the larva to the moth, provides a detailed analysis of the midgut of the adult insect, a stage that has been neglected up to now.

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Acknowledgments

This work was supported by a grant from the Italian Ministry of University and Research (PRIN 2008, protocol 2008SMMCJY) and by FAR 2013 (University of Insubria) to G.T. The authors wish to thank Dr. Makio Takeda for providing anti-CCAP antibody and Emanuela Mammoliti and Paola D'Antona for technical support.

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Authors and Affiliations

  1. Department of Biotechnology and Life Sciences, University of Insubria, Via J. H. Dunant, 3-21100, Varese, Italy

    Eleonora Franzetti, Davide Romanelli, Muthukumaran Rajagopalan, Annalisa Grimaldi, Magda de Eguileor & Gianluca Tettamanti

  2. Department of Biosciences, University of Milano, Via Celoria, 26, 20133, Milano, Italy

    Silvia Caccia & Morena Casartelli

  3. Department of Agriculture, University of Napoli “Federico II”, Via Università, 100, 80055, Portici, Napoli, Italy

    Silvia Caccia

  4. CRA – Honey Bee and Silkworm Research Unit, Padua seat, Via Eulero, 6A, 35143, Padova, Italy

    Silvia Cappellozza

  5. Department of Surgical and Morphological Sciences, University of Insubria, Via O. Rossi, 9, 21100, Varese, Italy

    Terenzio Congiu

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  1. Eleonora Franzetti
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  2. Davide Romanelli
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  3. Silvia Caccia
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  5. Terenzio Congiu
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  6. Muthukumaran Rajagopalan
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  7. Annalisa Grimaldi
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  8. Magda de Eguileor
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  9. Morena Casartelli
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  10. Gianluca Tettamanti
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Correspondence to Morena Casartelli or Gianluca Tettamanti.

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Franzetti, E., Romanelli, D., Caccia, S. et al. The midgut of the silkmoth Bombyx mori is able to recycle molecules derived from degeneration of the larval midgut epithelium. Cell Tissue Res 361, 509–528 (2015). https://doi.org/10.1007/s00441-014-2081-8

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