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Developmental regulations of Perp in mice molar morphogenesis

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Abstract

Teraspanin transmembrane protein, Perp (P53 apoptosis effector related to PMP22), which is found in the plasma membrane as a component of the desmosome, is reported to be involved in the morphogenesis of the epithelium and the enamel formation of the incisor. However, its expression pattern and signaling regulation during molar development have not been elucidated in detail. We have examined the precise expression patterns of Perp in developing lower molars and employed the knock-down of Perp by antisense oligodeoxynucleotide treatment during in vitro organ cultivation at embryonic day 13 to define the precise developmental function of Perp. Perp was expressed mainly in the dental lamina and stellate reticulum regions at the bud and cap stages. After Perp knock-down, the tooth germ showed disruption of the dental lamina and stellate reticulum with altered apoptosis and proliferation. The changed expression levels of related signaling molecules from the enamel knot and desmosome were evaluated by real-time quantitative polymerase chain reaction. A renal capsule transplantation method was employed to examine the effects of Perp knock-down on molar crown development. Ultrastructural observations revealed that enamel was deposited more densely in an irregular pattern in the cusp region, and that dentin was hypo-mineralized after Perp knock-down at the cap stage. Thus, Perp might play important roles in the formation and integration of stellate reticulum, dental lamina structure and enamel formation through signaling interactions with the enamel knot and desmosome-related signaling molecules at the cap stage of lower molar development.

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Authors and Affiliations

  1. Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 101 Dongin-dong 2ga, Jung-gu, Daegu, 700-422, South Korea

    Sanjiv Neupane, Wern-Joo Sohn, Ye-Ji Lee, Youngkyun Lee & Jae-Young Kim

  2. Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea

    Girdhari Rijal & Hong-In Shin

  3. School of Life Science and Biotechnology, Kyungpook National University, Daegu, South Korea

    Sanggyu Lee

  4. Department of Ultrastructural Science, Tokyo Dental College, Tokyo, Japan

    Hitoshi Yamamoto

  5. Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea

    Chang-Hyeon An

  6. Division of Anatomy and Developmental Biology, Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, South Korea

    Sung-Won Cho

  7. Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, Daegu, South Korea

    Tae-Yub Kwon

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  1. Sanjiv Neupane
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  2. Wern-Joo Sohn
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  3. Girdhari Rijal
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  7. Chang-Hyeon An
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  8. Sung-Won Cho
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  9. Youngkyun Lee
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  10. Hong-In Shin
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  11. Tae-Yub Kwon
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  12. Jae-Young Kim
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Corresponding author

Correspondence to Jae-Young Kim.

Additional information

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP; no. 2008–0062282).

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Figure S1

Immunolocalization of Perp. At E16.5, Perp is localized in the DL, SR and SI (a). Higher magnifications of images showing Perp localization in DL (b), SR (c) and SI (d). Similar localization pattern of Perp is observed in the OE, DL, SR and SI except the AB at PN2 and PN6 (e-l). OE; Oral Epithelium, DL; Dental Lamina, SR; Stellate Reticulum, SI; Stratum Intermedium, AB; Ameloblast. Scale bars 100 μm (a, e, i), 50 μm (b-c, f-g, j-k), 10 μm (d, h, l) (GIF 384 kb)

High resolution image (TIFF 6884 kb)

Figure S2

EDX-analysis (EDXA) of kidney capsule calcified control (a-c) and AS-ODN (d-f) teeth. The EDXA mapping images (b-c, e-f) for calcium and phosphorus distribution are based on SEM micrographs (a, d). The table shows the calcium and phosphorus distribution in the control and AS-ODN teeth. Ca; Calcium, P; Phosphorus (GIF 241 kb)

High resolution image (TIFF 8064 kb)

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Neupane, S., Sohn, WJ., Rijal, G. et al. Developmental regulations of Perp in mice molar morphogenesis. Cell Tissue Res 358, 109–121 (2014). https://doi.org/10.1007/s00441-014-1908-7

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  • DOI: https://doi.org/10.1007/s00441-014-1908-7

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