Abstract
The relationship between the type of retinal ganglion cell (RGC) and the retinoreceptive layer of the tectum is investigated by the immunostaining of RGCs with nicotinic acetylcholine receptorβ2 (nAChRβ2) antibody and intracellular staining by DiI and also by anterograde degeneration and biotinylated dextran amine labeling of retinotectal fibers in chicks. The results strongly suggest that many of the RGCs that express immunoreactivity to nAChRβ2 send axons to tectal layer 7 and are mainly classified into the simple-type of Groups II and III, which contain the cells providing middle-sized to large dendritic fields with simple dendritic arborization. These nAChRβ2-immunoreactive RGCs receive visual information via the multiple sublayers of the inner plexiform layer.
This is a preview of subscription content, log in via an institution to check access.
References
Bravo H, Pettigrew JD (1981) The distribution of neurons projecting from the retina and visual cortex to the thalamus and tectum opticum of barn owl, Tyto alba, and the burrowing owl, Speotyto cunicularia. J Comp Neurol 199:419–441
Butt CM, Pauly JR, Debski EA (2000) Distribution and development of nicotinic acetylcholine receptor subtypes in the optic tectum of Rana pipiens. J Comp Neurol 423:603–618
Cajal SR (1972) The structure of the retina (translated into English by Thorpe SA, Glickstein M). Thomas, Springfield
Chen Y, Naito J (2009) Morphological properties of chick retinal ganglion cells in relation to their central projections. J Comp Neurol 514:117–130
Crossland WJ, Uchwat CT (1979) Topographic projections of the retina and optic tectum upon the ventral geniculate nucleus in the chick. J Comp Neurol 185:87–106
Crossland WJ, Cowan WM, Kelly JP (1973) Observations on the transport of radioactivity labeled proteins in the visual system of the chick. Brain Res 56:77–105
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in proximal tubules of monkey kidney. Ultrastructural cytochemistry by a new technique. J Histchem Cytochem 14:291–302
Hamassaki-Britto DE, Brzozowska-Prechtl A, Karten HJ, Lindstrom JM, Keyser KT (1991) GABA-like immunoreactive cells containing nicotinic acetylcholine receptors in the chick retina. J Comp Neurol 313:394–408
Hayes BP (1982) The structural organization of the pigeon retina. Prog Retin Res 1:197–226
Henley JM, Lindstrom JM, Oswald RE (1986) Acetylcholine receptor synthesis in retina and transport to optic tectum in goldfish. Science 232:1627–1629
Hunt SP, Webster KE (1975) The projection of the retina upon the optic tectum of the pigeon. J Comp Neurol 162:433–446
Ikushima M, Watanabe M, Ito H (1986) Distribution and morphology of retinal ganglion cells in the Japanese quail. Brain Res 376:320–334
Keyser KT, Hughes TE, Whiting PJ, Lindstrom JM, Karten HJ (1988) Cholinoceptive neurons in the retina of the chick: an immunohistochemical study of the nicotinic acetylcholine receptors. Vis Neurosci 1:349–366
Kim TJ, Jeon CJ (2006) Morphological classification of parvalbumin—containing retinal ganglion cells in mouse: single-cell injection after immunocytochemistry. Invest Ophthalmol Vis Sci 47:2757–2764
Naito J, Chen Y (2004) Morphological analysis and classification of ganglion cells of the chick retina by intracellular injection of Lucifer yellow and retrograde labeling with DiI. J Comp Neurol 469:360–376
Oswald RE, Schmidt JT, Norden JJ, Freeman JA (1980) Localization of α-bungarotoxin binding sites to the goldfish retinotectal projection. Brain Res 187:113–127
Rager U, Rager G, Kabiersch A (1988) Transformations of the retinal topography along the visual pathway of the chicken. Anat Embryol 179:135–148
Remy M, Güntürkün O (1991) Retinal afferents to the tectum opticum and the nucleus opticus principalis thalami in the pigeon. J Comp Neurol 305:57–70
Thanos S, Vanselow J, Mey J (1992) Ganglion cells in the juvenile chick retina and their ability to regenerate axons in vitro. Exp Eye Res 54:377–391
Tömböl T (1998) Golgi and electron-microscopic Golgi-GABA immunostaining study of the avian optic tectum. Acta Anat 162:209–225
Whiting PJ, Schoepfer R, Conroy WG, Core MJ, Keyser KT, Shimasaki S, Esch F, Lindstrom JM (1991) Expression of nicotinic acetylcholine receptor subtypes in brain and retina. Mol Brain Res 10:61–70
Yamagata M, Sanes JR (1995) Target-independent diversification and target-specific projection of chemically defined retinal ganglion cell subsets. Development 121:3763–3776
Yamagata M, Weiner JA, Dulac C, Roth KA, Sanes JR (2006) Labeled lines in the retinotectal system: markers for retinorecipient sublaminae and the retinal ganglion cell subsets that innervate them. Mol Cell Neurosci 33:296–310
Acknowledgments
The authors thank Dr. Takayuki Watanabe at Teikyo University of Science for his kind and comprehensive support of this study.
Author information
Authors and Affiliations
Corresponding author
Additional information
Tanada and Watanabe contributed equally to this work.
This work was supported by a Grant-in-Aid for Scientific Research (C) (No. 20580331) (JSPS).
Rights and permissions
About this article
Cite this article
Naito, J., Tanada, Y. & Watanabe, T. Classification of nAChRβ2-immunoreactive retinal ganglion cells and their tectal projections in chicks. Cell Tissue Res 354, 881–886 (2013). https://doi.org/10.1007/s00441-013-1702-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00441-013-1702-y