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Cochlin expression in vestibular endorgans obtained from patients with Meniere’s disease

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Abstract

The distribution of cochlin and its associated basement membrane proteins (collagen IV, collagen II, laminin-β2, and nidogen-1) were evaluated in the vestibular endorgans of subjects with Meniere’s disease and compared with normal specimens. Cochlin mRNA expression in vestibular endorgans from Meniere’s disease specimens was also investigated. Specimens were obtained from patients who had Meniere’s disease and who were undergoing ablative labyrinthectomy. Control specimens were obtained both from autopsy specimens with documented normal audiovestibular function and from patients undergoing labyrinthectomy for acoustic neuroma excision. In the normal control specimens, cochlin immunoreactivity was found evenly distributed in the stroma of the cristae ampullaris and maculae of the utricle. In Meniere’s specimens, cochlin immunoreactivity was markedly increased; this was associated with an increase in cochlin mRNA expression as shown by real-time reverse transcription with the polymerase chain reaction. Collagen IV and laminin-β2 immunoreactivity was significantly decreased in Meniere’s specimens. Nidogen-1 and collagen II immunoreactivity was unchanged in Meniere’s specimens when compared with normal samples. Cochlin upregulation has been implicated in the hereditary audiovestibulopathy, DFNA9. The increased expression of cochlin and decreased expression of collagen IV and laminin in Meniere’s disease are suggestive that the overexpression of cochlin contributes to the dysfunctional inner ear homeostasis seen in this disease.

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Authors and Affiliations

  1. Department of Head and Neck Surgery, UCLA School of Medicine David Geffen, 10833 Le Conte Avenue, Los Angeles, CA, 90095, USA

    Audrey P. Calzada, Ivan A. Lopez & Akira Ishiyama

  2. Center of Cerebral Investigation, Veracruzana University, Veracruz, Mexico

    Luis Beltran Parrazal

  3. Neurology Department, UCLA School of Medicine David Geffen, 10833 Le Conte Avenue, Los Angeles, CA, 90095, USA

    Gail Ishiyama

  4. UCLA Department of Neurology, Reed Neurological Research Center at UCLA, Box 951769, 710 Westwood Blvd., Los Angeles, CA 90095, USA

    Gail Ishiyama

Authors
  1. Audrey P. Calzada
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  2. Ivan A. Lopez
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  3. Luis Beltran Parrazal
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  4. Akira Ishiyama
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  5. Gail Ishiyama
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Corresponding author

Correspondence to Gail Ishiyama.

Additional information

Meniere’s disease also known as Menière's or Ménière’s disease.

This work was supported by National Institutes of Health grant 5U24 DC 008635 (NIDCD).

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Calzada, A.P., Lopez, I.A., Beltran Parrazal, L. et al. Cochlin expression in vestibular endorgans obtained from patients with Meniere’s disease. Cell Tissue Res 350, 373–384 (2012). https://doi.org/10.1007/s00441-012-1481-x

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  • DOI: https://doi.org/10.1007/s00441-012-1481-x

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