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The novel expression of Oct3/4 and Bmi1 in the root development of mouse molars

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Abstract

The root apex of the tooth elongates until the completion of root development. Although the signaling molecules inducing root elongation have been studied, the characteristic of the cells having the ability to maintain the root elongation remains unclear. This study aimed to investigate the characteristics of the cells involved in the root elongation. Octamer-binding factor 3/4 (Oct3/4) is known as one of the key regulators in maintaining the pluripotency and self-renewal properties of embryonic stem cells. Bmi1, the polycomb-group transcriptional repressor, has emerged as a key regulator in several cellular processes including stem cell self-renewal and cancer cell proliferation. At the beginning of root formation, ameloblasts expressed Oct3/4 in the nucleus, except in the apex of the cervical loop, in which Bmi1and cyclinD were expressed. At PN6, the expression of Oct3/4 in the ameloblasts shifted from the nucleus to the cytoplasm, whereas ameloblastin-negative Hertwig’s epithelial root sheath (HERS) cells expressed Bmi1 and cyclinD. By PN10, the cells in the apex of HERS began to express Oct3/4 in their nucleus, whereas Bmi1 and cyclinD began to decrease in their expressions. The odontoblasts consistently expressed Oct3/4 in their cytoplasm. Our results suggest that (1) Oct3/4 creates the border between the ameloblasts from the proliferative region of HERS, (2) Bmi1-positive cells would be one of the candidates resulting in root elongation and (3) the Oct3/4 expression in the cytoplasm of odontoblasts may be related to maintain the odontoblastic characteristics.

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Acknowledgments

This work was supported by National Research Foundation of Korea (NFR) grant funded by the Korea government (MEST) (No. 2011-0015661).

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Authors and Affiliations

  1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korean 21 project, Oral Science Research Center, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-Gu, Seoul, 120-752, Korea

    Eizo Nakagawa, Li Zhang, Jeong-Oh Shin, Eun-Jung Kim, Sung-Won Cho & Han-Sung Jung

  2. State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China, 430079

    Li Zhang & Zhi Chen

  3. Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Hayato Ohshima

Authors
  1. Eizo Nakagawa
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  2. Li Zhang
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  3. Jeong-Oh Shin
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  4. Eun-Jung Kim
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  6. Hayato Ohshima
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  7. Zhi Chen
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  8. Han-Sung Jung
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Corresponding authors

Correspondence to Zhi Chen or Han-Sung Jung.

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Eizo Nakagawa and Li Zhang contributed equally to this work.

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Nakagawa, E., Zhang, L., Shin, JO. et al. The novel expression of Oct3/4 and Bmi1 in the root development of mouse molars. Cell Tissue Res 347, 479–484 (2012). https://doi.org/10.1007/s00441-011-1310-7

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  • DOI: https://doi.org/10.1007/s00441-011-1310-7

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