Abstract
This study investigated the effect of adding different insulin concentrations to the culture medium for goat preantral follicle development in vitro. The ovarian fragments were immediately fixed or cultured for 7 days in MEM with insulin (0, 5, 10 ng/ml and 5 or 10 μg/ml). The results showed that, after 7 days of culture, insulin at 10 ng/ml was the best concentration to preserve follicular viability and ultrastructure, resulting in the highest rates of normal follicles. After 7 days, only treatments with 10 ng/ml and 5 μg/ml of insulin increased follicular activation when compared to other concentrations. Regarding follicular and oocyte growth, the presence of 10 ng/ml of insulin promoted a larger diameter than other treatments. In conclusion, this study shows that addition of 10 ng/ml of insulin to the culture medium improved the survival and stimulated growth of goat preantral follicles.
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Acknowledgments
This work was supported by CNPq (RENORBIO). R.N. Chaves is a recipient of a grant from CNPq (Brazil). The authors thank Khesller Patricia Olazia Name for her assistance in processing the samples for the ultrastructural assessment.
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Chaves, R.N., Alves, A.M.C.V., Faustino, L.R. et al. How the concentration of insulin affects the development of preantral follicles in goats. Cell Tissue Res 346, 451–456 (2011). https://doi.org/10.1007/s00441-011-1264-9
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DOI: https://doi.org/10.1007/s00441-011-1264-9