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Dynamics of expression of ARID1A and ARID1B subunits in mouse embryos and in cells during the cell cycle

Cell and Tissue Research volume 345, pages 137–148 (2011)Cite this article

Abstract

The mammalian SWI/SNF chromatin remodeling complexes play essential roles in cell cycle control through the transcriptional regulation of cell-cycle-specific genes. These complexes depend on the energy of ATP hydrolysis provided by the BRG1 or BRM catalytic subunit. They contain seven or more noncatalytic subunits, some being constitutive components, with others having paralogs that assemble in a combinatory manner producing different SWI/SNF-related complexes with specific functions. ARID1A and ARID1B are mutually exclusive subunits of the BAF complex. The specific presence of these subunits in the complex has been demonstrated to determine whether SWI/SNF functions as a corepressor (ARID1A) or as a coactivator (ARID1B) of the cell cycle genes. Our aim has been to analyze the relevance of the ARID1 subunits in development. We have compared the patterns of expression of these two genes through various mouse embryonic stages. Arid1a is expressed widely and intensively, whereas Arid1b is poorly transcribed and expressed in selected regions. Moreover, ARID1A and ARID1B present different kinetics of expression in the cell cycle. ARID1A accumulates in G0 and is downregulated throughout the cell cycle phases but is completely eliminated during mitosis, whereas ARID1B is expressed at comparable levels at all phases, even during mitosis. These kinetics probably affect the incorporation patterns of the ARID1 proteins to the complex and hence modulate SWI/SNF activity during proliferation and arrest.

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Acknowledgements

We thank Andrés Saralegui for assistance with the confocal microscopy, Dr. Enrique Salas for assistance in work with preimplantation embryos, Aimée Bastidas and David Hernández for assistance with ES culture, María Elena Bravo-Adame and Erika Melchy for assistance in the cell sorting, and Marcela Ramírez for assistance with mice.

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Affiliations

  1. Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Angel Flores-Alcantar, Adriana Gonzalez-Sandoval & Hilda Lomelí

  2. División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico

    Diana Escalante-Alcalde

Authors
  1. Angel Flores-Alcantar
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  2. Adriana Gonzalez-Sandoval
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  3. Diana Escalante-Alcalde
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  4. Hilda Lomelí
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Corresponding author

Correspondence to Hilda Lomelí.

Additional information

This work was supported by DGAPA-UNAM grant IN220009-3 and CONACyT 49114.

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Flores-Alcantar, A., Gonzalez-Sandoval, A., Escalante-Alcalde, D. et al. Dynamics of expression of ARID1A and ARID1B subunits in mouse embryos and in cells during the cell cycle. Cell Tissue Res 345, 137–148 (2011). https://doi.org/10.1007/s00441-011-1182-x

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  • DOI: https://doi.org/10.1007/s00441-011-1182-x

Keywords

  • ARID
  • Chromatin remodeling
  • Cell cycle
  • Developmental expression
  • BAF complex
  • Mouse (CD1)