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Spatiotemporal expression profile of no29/nucleophosmin3 in the intestine of Xenopus laevis during metamorphosis

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Abstract

A Xenopus laevis homolog of nucleophosmin/nucleoplasmin3 (NPM3), no29, has been previously identified as a thyroid hormone (TH)-response gene during TH-induced metamorphosis. X. laevis has another NPM3 homolog (npm3) in the pseudo-tetraploid genome, whereas X. tropicalis possesses one ortholog in the diploid genome. To assess the possible roles of these NPM3 homologs in amphibian metamorphosis, we have analyzed their expression profiles in X. laevis tadpoles. Levels of no29 and npm3 mRNA are rapidly up-regulated by exogenous TH in various organs of the premetamorphic tadpoles. Notably, in the small intestine, no29 and npm3 mRNA levels are transiently up-regulated during metamorphic climax, when progenitor/stem cells of the adult epithelium appear and actively proliferate. In situ hybridization analysis has revealed that the no29 transcript is specifically localized in adult epithelial progenitor/stem cells of the intestine during natural and TH-induced metamorphosis. Double-staining for in situ hybridization and immunohistochemistry has shown co-expression of no29 mRNA and no38 protein (an ortholog of NPM1), which is known to interact with NPM3 and to regulate cell proliferation in mammals. Thus, no29/npm3 might serve as a stem cell marker in the intestine during metamorphosis.

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Acknowledgements

We thank Mrs. Mitsuko Kajita (Nippon Medical School) for her technical help. Part of this research was performed when K.S. and N.M. were affiliated to the Graduate School of Science, Hiroshima University, Japan.

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Authors and Affiliations

  1. Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima, 939–8526, Japan

    Natsuki Motoi

  2. Department of Biology, Nippon Medical School, 2-297-2 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa, 211–0063, Japan

    Takashi Hasebe & Atsuko Ishizuya-Oka

  3. Center for Marine Environmental Studies, Ehime University, 2–5 Bunkyo-cho, Matsuyama, Ehime, 790–8577, Japan

    Ken-ichi T. Suzuki

Authors
  1. Natsuki Motoi
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  2. Takashi Hasebe
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  3. Ken-ichi T. Suzuki
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  4. Atsuko Ishizuya-Oka
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Corresponding author

Correspondence to Ken-ichi T. Suzuki.

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Natsuki Motoi and Takashi Hasebe contributed equally to this work.

This study was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) to K.S. and A.I.-O., a Narishige Zoological Science Award to T.H., and a grant from the Naito Foundation to K.S. Additional support by the “Global COE Program” of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan is gratefully acknowledged by K.S.

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Motoi, N., Hasebe, T., Suzuki, Ki.T. et al. Spatiotemporal expression profile of no29/nucleophosmin3 in the intestine of Xenopus laevis during metamorphosis. Cell Tissue Res 344, 445–453 (2011). https://doi.org/10.1007/s00441-011-1163-0

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