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Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio)

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Abstract

Calbindin is a calcium-binding protein, commonly found in certain subpopulations of the enteric nervous system in mammals. Recently, calbindin-immunoreactive enteric neurons have also been demonstrated in shorthorn sculpin (Myoxocephalus scorpius). In the present study, calbindin immunoreactivity has been investigated in the gut of adult and larval zebrafish (Danio rerio) and differences and similarities between the two species are discussed. Calbindin immunoreactivity is present in 40%–50% of all enteric neurons in adult zebrafish. It first appears at 3 days post-fertilisation (dpf) and is present in all regions of the gut by 13 dpf. Calbindin-immunoreactive nerve cell bodies do not differ in size from calbindin-negative cells. Zebrafish calbindin-immunoreactive neurons are serotonin-negative, with at least some being choline acetyltransferase (ChAT)-positive, in contrast to the sculpin in which cells are generally smaller than the average enteric neuron and are serotonin-positive and ChAT-negative. These findings further emphasise the importance of comparative studies for understanding the diversity of chemical coding in the enteric nervous system of fish and other vertebrates. Improved knowledge of the role of the enteric nervous system is also essential for future studies of gut activity with regard to zebrafish being used as a model organism.

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Acknowledgement

The author thanks Ms. Christina Hagström for expert technical assistance with the immunohistochemistry and rearing of zebrafish larvae.

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Authors and Affiliations

  1. Department of Zoology/Zoophysiology, University of Gothenburg, Box 463, SE 405 30, Göteborg, Sweden

    Catharina Olsson

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  1. Catharina Olsson
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Correspondence to Catharina Olsson.

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This study was supported by the Swedish Research Council (grant 2007-6105).

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Olsson, C. Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio). Cell Tissue Res 344, 31–40 (2011). https://doi.org/10.1007/s00441-011-1135-4

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